测定尿中对硝基酚(PNP)含量常用的比色法,其最低检出浓度为0.1ppm,在PNP含量较低时,需用大量尿标本(100ml),而且特异性较差。Cranmer提出在色谱柱中PNP形成三甲基硅烷基醚的衍生物进行气相包谱分析,可测定人尿中0.05ppm的PNP。这个方法用于测定大鼠尿中的PNP,需要在注入电子捕获鉴定器以前除去干扰物质;而且硅烷基醚衍 Colorimetric method commonly used for the determination of p-nitrophenol (PNP) in urine has a minimum detectable concentration of 0.1ppm. At low PNP levels, a large amount of urine sample (100ml) is required and the specificity is poor. Cranmer proposed PNP formation of trimethylsilyl ether derivatives in the column for gas-phase spectral analysis, which determined 0.05 ppm of PNP in human urine. This method is used to determine PNP in rat urine and requires the removal of interfering substances prior to injection into the electron capture detector;