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为了剔除矮脚鸡群中的杂合子个体,使其在后代繁殖过程中无性状分离,鸡群脚胫和体重等方面的均匀度达到一致。试验采用2对特定引物对64只矮脚黄羽鸡进行PCR扩增,对扩增片段进行测序确定。结果表明:有51只矮小纯合子鸡,纯合子频率为79.69%,其余13只为矮小杂合子鸡,杂合子频率为20.31%。说明利用特定的引物对矮小鸡群体进行PCR扩增筛选能有效剔除矮脚鸡群中的杂合子个体,从而加快育种进程。
In order to eliminate the heterozygous individuals in the bantam group, it has the same uniformity in the segregation of the fowls and the weight of the fowls during the reproduction of the offspring. Two pairs of specific primers were used for PCR amplification of 64 dwarf yellow chickens, and the amplified fragments were sequenced. The results showed that there were 51 short homozygous chickens. The frequency of homozygote was 79.69%, and the remaining 13 were short heterozygous chickens. The frequency of heterozygote was 20.31%. It shows that using specific primers to screen the chicken population for PCR amplification can effectively eliminate heterozygous individuals in the bantam group and accelerate the breeding process.