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Objective: To investigate the inhibitory effects of RNAi (RNA interference, RNAi) expression vector on CXCR4 expression in prostate carcinoma cell lines. Methods: Small interference RNA(siRNA) expression vectors for CXCR4 gene were constructed and transfected into prostate carcinoma cell lines(PC-3m and LNCaP)with liposomes. The expression of CXCR4 was detected by RT-PCR and western blot. Results: The expression of CXCR4 mRNA and protein in the PC-3m and LNCaP cells was reduced by RNAi expression vectors. The inhibitory rate of CXCR4 mRNA expression in the PC-3m cells was 87.81%±10.20%,56.10%±9.32% at the 24th hour and the 48th hour, compared with 56.93%±8.78%,49.24%±11.23% in LNCaP cells. The inhibitory rate of the expression of CXCR4 protein was 64.71%±6.68%,58.66%±11.56% respectively. Conclusion: The expression of CXCR4 gene can effectively be inhibited by RNAi expression vectors.
Objective: To investigate the inhibitory effects of RNAi (RNAi) expression vector on CXCR4 expression in prostate carcinoma cell lines. Methods: Small interference RNA (siRNA) expression vectors for CXCR4 gene were constructed and transfected into prostate carcinoma cell lines -3m and LNCaP) with liposomes. The expression of CXCR4 was detected by RT-PCR and western blot. Results: The expression of CXCR4 mRNA and protein in the PC-3m and LNCaP cells was reduced by RNAi expression vectors. The inhibitory rate of CXCR4 mRNA expression in the PC-3m cells was 87.81% ± 10.20%, 56.10% ± 9.32% at the 24th hour and the 48th hour, compared with 56.93% ± 8.78%, 49.24% ± 11.23% in LNCaP cells. of the expression of CXCR4 protein was 64.71% ± 6.68%, 58.66% ± 11.56% respectively. Conclusion: The expression of CXCR4 gene can be effectively inhibited by RNAi expression vectors.