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本文采用二株鼠抗人胃癌单克隆抗体(单抗)MG_7、MGd_1混合后,用双抗体夹心ELBA法对65例胸腹水中MG_7-Ag、MGd_1-Ag的表达情况进行检测。结果表明:胃肠癌患者胸腹水中MG_7-Ag与MGd_1-Ag检测的阳性率为50％,肺癌为47.6％,而卵巢癌、宫颈癌的良性疾病患者的胸腹水则为阴性。实验结果提示:胸腹水中MG_7-Ag、MGd_1-Ag的检测对肿瘤性胸腹水的诊断及鉴别诊断具有一定价值。
In this study, two mouse anti-human gastric cancer monoclonal antibodies (mAb) MG_7 and MGd_1 were mixed, and the expression of MG_7-Ag and MGd_1-Ag in 65 cases of pleural effusion and ascites fluid was detected by double antibody sandwich ELBA. The results showed that the positive rates of MG_7-Ag and MGd_1-Ag in thoracic and abdominal fluids of patients with gastrointestinal cancer were 50% and 47.6% in lung cancer. The pleural effusion and ascites in patients with benign diseases of ovarian and cervical cancer were negative. The experimental results suggest that the detection of MG_7-Ag and MGd_1-Ag in thoracic and abdominal fluids is valuable for the diagnosis and differential diagnosis of tumor-induced pleural effusion and ascites.