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目的:观察氨基胍(AG)对体外培养的人翼状胬肉成纤维细胞增殖的影响。方法:取人翼状胬肉标本进行体外贴壁细胞常规培养,传代至第3～5代细胞用于试验,以每孔1×104个细胞接种于培养板中,加入不同浓度(250、500、750、1000μmol.L-1)的AG作为AG组,另设不加AG的为对照组,检测作用24、48、72h后各孔的吸光度,并计算细胞增殖抑制率,每个时间点、每个浓度均设6个复孔。结果:人翼状胬肉成纤维细胞增殖抑制率随AG浓度的增大和作用时间的延长明显升高(P<0.05)。结论:AG在受试浓度和受试时间范围内,对体外培养的人翼状胬肉成纤维细胞具有抑制作用且呈浓度、时间依赖性。
Objective: To observe the effect of aminoguanidine (AG) on the proliferation of cultured human pterygium fibroblasts in vitro. Methods: The specimens of human pterygium were collected and cultured in vitro. The cells were passaged to the third to fifth passage for testing. The cells were inoculated into the culture plate with 1 × 104 cells per well. Different concentrations (250, 500, 750,1000μmol.L-1) AG as the AG group, another set without AG as the control group, 24, 48, 72h after the detection of the absorbance of each well, and calculate the inhibition rate of cell proliferation, each time point, each A concentration of 6 complex holes. Results: The inhibitory rate of human pterygium fibroblast proliferation was significantly increased with the increase of AG concentration and prolongation of action time (P <0.05). CONCLUSION: AG inhibits human pterygium fibroblasts cultured in vitro in a concentration-and time-dependent manner at the concentrations tested and over the test period.