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将月季Rosa odorata Var erubescens Rehd.et Wils的单芽茎段接种在MS或改良MS培养基上培养,在附加BA(或KT)0.1—0.5ppm,IAA0.05ppm的培养基上,5—7天腋芽萌发,15天节间伸长成枝条40,天左右每芽位形成枝条2—3个。将萌发后长出3—5片叶的单芽代部分茎段组织转到附加BA2—3ppmN,AA0.005—0.01ppm的培养基上,56天左右可分化出12个左右的丛生芽。将丛生芽切割后转到附加BA0.25—0.5ppm,IAA0.01—0.05ppm的培养基上,形成的枝条木质化程度好,剪下转入含NAA0.05—0.5PPM的培养基上诱出根后移出试管成活率高。在20—30℃范围内诱根时,NAA的浓度随温度升高而降低,否则出现愈伤组织过多,影响试管苗的移栽成活率。
The single shoots of Rosa odorata var erubescens Rehd.et Wils were inoculated on MS or modified MS medium and cultured on medium supplemented with BA (or KT) 0.1-0.5ppm and IAA 0.05ppm for 5-7 days Axillary bud germination, 15-day internode elongation into branches 40, about 2-3 days per sprout formation of branches. The germination will grow 3-5 leaves single shoot on behalf of some stem tissue to BA2-3ppmN, AA0.005-0.01ppm additional medium, about 56 days can differentiate about 12 clusters of buds. The cluster shoots were cut and transferred to additional BA0.25-0.5ppm, IAA0.01-0.05ppm medium, the degree of lignification of the branches formed is good, cut into medium containing NAA0.05-0.5PPM lure Out of the root after the tube out of high survival rate. In the range of 20-30 ℃ induced roots, the concentration of NAA decreased with increasing temperature, or there is too much callus, affecting the survival rate of transplanted in vitro plantlets.