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目的：研究重组人肿瘤坏死因子（rhTNF－α）对鼻咽癌细胞敏感性、重组人白细胞介素－2（rhIL－2）协同抗瘤效应及抗瘤机理。方法：利用我室建立的鼻咽癌细胞株（CNE3）制成的裸鼠鼻咽癌模型，rhTNFα通过与rhIL－2配伍瘤内注射以观察移植瘤超微结构及免疫组织化学的改变，探讨其作用机理。结果：鼻咽癌裸鼠移植瘤经治疗后出现坏死、体积缩小、甚至消失；超微结构观察凋亡细胞缩皱，核内异染色质边集；免疫组化研究发现：rhTNF－α有下调（突变型）p53和增殖细胞核抗原（PCNA）蛋白表达，其可能与rhTNF－α的抗癌机理有关；rhIL－2能增强rhTNF－α的抗瘤作用。结论：rhIL－2增强rhTNF－α的抗瘤机理可能与IL－2诱导癌细胞表达TNF受体作用有关，该研究为临床在鼻咽镜下进行TNF局部治疗提供实验依据。
Objective: To study the sensitivity of recombinant human tumor necrosis factor (rhTNF-α) on nasopharyngeal carcinoma cells, the synergistic antitumor effect of recombinant human interleukin-2 (rhIL-2), and the mechanism of anti-tumor. METHODS: The nude mouse nasopharyngeal carcinoma model established by CNE3 in our department was used to investigate the ultrastructural and immunohistochemical changes of transplanted tumors by intratumoral injection of rhTNFα and rhIL-2. Its mechanism of action. RESULTS: The neoplasm transplanted in nude mice developed necrosis, shrinkage or even disappeared after treatment. The ultrastructure was observed for atrophy of apoptotic cells and nuclear heterochromatin. The immunohistochemical study found that rhTNF-α was down-regulated. (Mutant) p53 and proliferating cell nuclear antigen (PCNA) protein expression, which may be related to the anti-cancer mechanism of rhTNF-α; rhIL-2 can enhance the anti-tumor effect of rhTNF-α. Conclusion: The anti-tumor mechanism of rhIL-2 enhancing rhTNF-α may be related to the effect of IL-2 on the expression of TNF receptor in cancer cells. This study provides experimental basis for the local treatment of TNF under the nasopharyngeal microscope.