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目的建立雌性大鼠性唤起阴道血流电生理研究模型,确定电生理学参数,研究发情周期不同阶段对模型阴道血流的影响。方法雌性Wistar大鼠30只,分为A组(n=10)和B组(n=20只)。A组大鼠以电流刺激盆神经阴道支(PNS),利用BIOPAC MPl00电生理仪测定不同频率电流刺激下,阴道血流变化(电压6V、脉冲间隔0．8ms,刺激持续时问30 s,刺激间歇期为5分钟,刺激频率设定为2．5Hz、5Hz、10 Hz和20Hz)。B组大鼠,通过阴道涂片确定发情周期不同阶段,PNS下测定阴道血流变化,比较发情周期不同阶段阴道血流差异。结果10Hz电流(6V、0．8ms、30s)PNS下,阴道血流反应较灵敏且对动脉血压没有影响,便于进行性唤起过程中阴道血流研究。固定参数PNS下(6V、0．8ms、30 s、10Hz),发情周期不同阶段雌性大鼠阴道血流峰值和曲线下面积未见明显变化(P＞0．05)。结论雌性大鼠可作为性唤起阴道血流电生理研究动物模型,确定10Hz、电压6V、脉冲间隔0．8ms、刺激持续时间30s为电刺激时的固定参数。大鼠性周期不同阶段PNS下大鼠阴道血流无明显差异,便于进行性唤起过程中雌性大鼠阴道血流研究。
Objective To establish an electrophysiological study model of sexual arousal vaginal blood flow in female rats to determine electrophysiological parameters and study the influence of different stages of estrus cycle on vaginal blood flow. Methods Thirty female Wistar rats were divided into group A (n = 10) and group B (n = 20). A group of rats to stimulate the vaginal branch of the pelvis nerve (PNS), the use of BIOPAC MPl00 electrophysiometer measured different frequency current stimulation, vaginal blood flow changes (voltage 6V, pulse interval of 0.8ms, the duration of the stimulus for 30s, stimulation Intermittent period of 5 minutes, the stimulation frequency is set to 2.5Hz, 5Hz, 10Hz and 20Hz). Group B rats, vaginal smear determination of estrous cycle in different stages, PNS vaginal blood flow changes measured during different stages of estrous cycle vaginal blood flow differences. Results 10Hz current (6V, 0.8ms, 30s) PNS, the vaginal blood flow response is sensitive and has no effect on arterial blood pressure, facilitate the process of vaginal blood flow during sexual arousal. Fixed parameters PNS (6V, 0.8ms, 30s, 10Hz), estrous cycle in different stages of female rats vaginal blood flow peak area and under the curve showed no significant change (P> 0.05). Conclusion Female rats can be used as animal model of electrophysiological evoked vaginal blood flow to determine the fixed parameters of 10Hz, voltage 6V, pulse interval 0.8ms, duration of stimulation 30s for electrical stimulation. There was no significant difference in vaginal blood flow between PNS rats in different stages of the sexual cycle of rats, which is helpful to study vaginal blood flow in female rats during sexual arousal.