Objective:The incidence of after-cataracts [also known as posterior capsular opacification (PCO)] is between 30% and 50% three years following cataract surgery.Suppressing the proliferation of lens epithelial cells (LECs) is a primary goal in preventing PCO.Here,we investigated the proteomic regulation of the inhibitory effects of curcumin (Cur) on the proliferation of human lens epithelial B3 (HLE-B3) cells.Methods:Recombinant human basic fibroblast growth factor (rhbFGF) was used to induce proliferation of HLE-B3 cells,which were incubated with 20 mg/L Cur in a CO 2 incubator for 24 h.Results:We found that the absorbance (A) value of rhbFGF group was significantly higher than the A value of the control group.Furthermore,the A value of the Cur group was significantly lower compared to the rhbFGF group,with an inhibition of 53.7%.Five different protein spots were obtained from proliferative HLE-B3 cells induced by rhbFGF.Eight different protein spots were obtained in HLE-B3 cells incubated with Cur.There were the common variational protein spots at mass/charge (m/z) ratios of 8093 and 13767 between rhbFGF group and control group as well as between the Cur group and rhbFGF group.Conclusions:These results show that Cur effectively inhibited HLE-B3 cell proliferation induced by rhbFGF.The protein spots at m/z of 8093 and 13767 may be the targets of Cur-induced inhibition of HLE-B3 cell proliferation.Cur may be a reliable and effective drug for prevention and treatment of polymerase chain reaction (PCR). Objective: The incidence of after-cataracts [also known as posterior capsular opacification (PCO)] is between 30% and 50% three years following cataract surgery. Suppressing the proliferation of lens epithelial cells (LECs) is a primary goal in prevented PCO. Here, we investigated the proteomic regulation of the inhibitory effects of curcumin (Cur) on the proliferation of human lens epithelial B3 (HLE-B3) cells. Methods: Recombinant human basic fibroblast growth factor (rhbFGF) was used to stimulate proliferation of HLE- B3 cells, which were incubated with 20 mg / L Cur in a CO2 incubator for 24 h. Results: We found that the absorbance (A) value of rhbFGF group was significantly higher than the A value of the control group. Futurertherm, the A value of the Cur group was significantly lower compared to the rhbFGF group, with an inhibition of 53.7%. Five different protein spots were obtained from proliferative HLE-B3 cells induced by rhbFGF. Different different protein spots were obtained in HLE-B3 cells ated with Cur. There were the common variational protein spots at mass / charge (m / z) ratios of 8093 and 13767 between rhbFGF group and control group as well as the cur group and rhbFGF group. inhibited HLE-B3 cell proliferation induced by rhbFGF.The protein spots at m / z of 8093 and 13767 may be the targets of Cur-induced inhibition of HLE-B3 cell proliferation. Cur may be a reliable and effective drug for prevention and treatment of polymerase chain reaction (PCR).