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为观察低血钙对神经元内钙结合蛋白的影响，用免疫细胞化学法，研究了低血钙大鼠脊髓腹角内钙结合蛋白（ＣａＢＰ）免疫反应（ＣａＢＰ＼｜ＩＲ）神经元的变化。正常情况下，大鼠脊髓腹角内ＣａＢＰ－ＩＲ神经元较少，主要位于运动神经元的腹侧，为一些中小型神经元。低血钙４周以上的大鼠，腹角内阳性细胞的数量和纤维密度显著增加。此时，阳性神经元明显分为３群：内侧群位于腹角的内侧，多为一些中等大小的梭形细胞；背侧群位于运动神经元的背侧，多为大多角形神经元；腹侧群位于外侧运动柱的内侧或运动神经元之间，为中等大小的神经元，部分大多极神经元也呈现阳性。本研究结果显示低血钙可使脊髓腹角内神经元合成ＣａＢＰ增加
To observe the effect of hypocalcemia on intracellular calcium-binding protein in neurons, we used immunocytochemistry to study the changes of calcium-binding protein (CaBP) immunoreactivity (CaBP) | IR in the ventral horn of hypocalcemic rats . Normally, there are fewer CaBP-IR neurons in the ventral horn of spinal cord of rats, which are mainly located in the ventral part of motor neuron and are some small and medium-sized neurons. Hypocalcemia in rats more than 4 weeks, the ventral horn of the number of positive cells and fiber density increased significantly. At this point, the positive neurons were apparently divided into three groups: the medial group located in the ventral medial, mostly for some medium-sized spindle cells; dorsal group located in the dorsal motor neurons, mostly large polygonal neurons; ventral The group is located inside the lateral movement of the column or between motor neurons, medium-sized neurons, some of the majority of polar neurons also showed positive. The results of this study show that hypocalcemia can increase the synthesis of CaBP in neurons in the ventral horn of the spinal cord