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目的研究补体对RAW264.7小鼠巨噬细胞吞噬阿萨希毛孢子菌(Trichosporon asahii,T.asahii)的增强作用。方法实验分为3组,实验组为T.asahii菌液加入10%正常小鼠血清37℃预孵育1h。空白对照组为菌液不经正常小鼠血清预孵育。热灭活对照组为正常小鼠血清预先在56℃水浴箱孵育30min,然后加入T.asahii菌液37℃预孵育30min。3组预处理的T.asahii菌液加入RAW264.7巨噬细胞共培养30min后在显微镜下观察RAW264.7细胞吞噬T.asahii的情况,计数吞噬个数及吞噬率。结果实验组与空白对照组及热灭活对照组的吞噬率比较,差异均具有显著性(P<0.01)。空白对照组与热灭活对照组的吞噬率比较,无显著性差异(P>0.05)。结论补体可增强RAW264.7小鼠巨噬细胞吞噬T.asahii的作用。
Objective To study the enhancement effect of complement on phagocytosis of Trichosporon asahii (T.asahii) by RAW264.7 mouse macrophages. Methods The experiment was divided into three groups, experimental group was T.asahii bacterial solution, 10% normal mouse serum was pre-incubated for 1 hour at 37 ℃. The blank control group was not pre-incubated with normal mouse serum. Heat-inactivated control group of normal mouse serum pre-incubated in a 56 ℃ water bath for 30min, then add T.asahii bacteria solution pre-incubated at 37 ℃ for 30min. Three groups of pretreated T.asahii bacteria were added to RAW264.7 macrophages co-cultured for 30min after the microscope to observe the RAW264.7 cells phagocytosed T.asahii situation, the number of phagocytosis and phagocytosis rate. Results The phagocytic rates of experimental group, blank control group and heat-inactivated control group were significantly different (P <0.01). The phagocytic rate of the blank control group and the heat-inactivated control group showed no significant difference (P> 0.05). Conclusion Complement can enhance the phagocytosis of T.asahii by RAW264.7 mouse macrophages.