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目的观察IR模型大鼠视黄醇结合蛋白4(RBP4)和8-羟基脱氧鸟苷(8-OHdG)表达水平及运动和吡格列酮的干预作用。方法 40只雄性Wistar大鼠随机分为对照(NC)组和模型(M)组,分别给予普通和高糖高脂饲料,8周后再将M组随机分为IR组、运动(Exercise)组和吡格列酮(Pio)组,后2组分别进行游泳训练及吡格列酮灌胃,持续8周。16周末采用稳态模型评估胰岛素抵抗指数(HOMAIR)、检测血清RBP4、骨骼肌超氧化物歧化酶(SOD)及8-OHdG水平。结果 (1)IR组HOMA-IR、RBP4升高,8-OHdG增强(P<0.01),SOD降低(P<0.01);Exercise、Pio组HOMA-IR、RBP4和8-OHdG下降,SOD增加(P<0.05)。(2)相关性分析发现,HOMA-IR与RBP4、8-OHdG呈正相关(r=0.690、0.628,P<0.01),与SOD呈负相关(r=-0.712,P<0.01);RBP4与8-OHdG呈正相关(r=0.656,P<0.01),与SOD呈负相关(r=-0.649,P<0.01)。结论 RBP4可能通过增加IR大鼠氧化应激参与IR,运动、吡格列酮可能通过抑制IR大鼠氧化应激,降低RBP4改善IR。
Objective To observe the expression of retinol binding protein 4 (RBP4) and 8-hydroxydeoxyguanosine (8-OHdG) and the effects of exercise and pioglitazone on IR rats. Methods Forty male Wistar rats were randomly divided into control group (NC) and model group (M), and were given normal and high glucose and high fat diet respectively. After 8 weeks, M group was randomly divided into IR group, Exercise group And pioglitazone (Pio) group, the latter two groups were swimming training and pioglitazone gavage for 8 weeks. At 16th weekend, the homeostasis model was used to assess the insulin resistance index (HOMAIR), serum RBP4, skeletal muscle superoxide dismutase (SOD) and 8-OHdG levels. Results Compared with control group, the levels of HOMA-IR, RBP4 and 8-OHdG increased (P <0.01) and decreased in Exercise and Pio group (P <0.01) P <0.05). (2) Correlation analysis showed that there was a positive correlation between HOMA-IR and RBP4,8-OHdG (r = 0.690,0.628, P <0.01) and negative correlation with SOD -OHdG (r = 0.656, P <0.01), but negatively correlated with SOD (r = -0.649, P <0.01). Conclusion RBP4 may participate in IR and exercise by increasing oxidative stress in IR rats. Pioglitazone may reduce oxidative stress in IR rats and reduce RBP4 to improve IR.