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在白皮松主要分布区抽取了 10个天然群体进行酶电泳分析 ,共测定了 16种酶系统 ,得到 31个清晰酶位点和 5 3个等位基因。按照基因频率进行分类 ,5 3个基因中有 32个全局基因、14个广域基因、6个局域基因和 1个特异基因。通过计算机模拟建立基因捕获曲线的结果表明 ,随机抽取 5个群体可平均捕获 99.9%的基因。群体的等位基因频率与地理生态因子的相关分析表明 ,Idh与Pgi 2两个位点的基因频率呈现明显的梯度变异。该项研究为有效保护白皮松天然群体的基因资源提供了依据
In the main distribution of Pinus bungeana, 10 natural populations were extracted for enzyme electrophoresis analysis. A total of 16 enzyme systems were assayed and 31 clear enzyme sites and 53 alleles were obtained. According to the gene frequency classification, there were 32 global genes among 5 3 genes, 14 wide-area genes, 6 local genes and 1 specific gene. The results of computer simulation to establish the gene trapping curve showed that 5 randomly selected groups could capture 99.9% of the genes on average. Correlation analysis between allele frequency and geographical ecological factors showed that the frequency of Idh and Pgi 2 showed obvious gradient variation. This study provides the basis for effectively protecting the genetic resources of Pinus bungeana natural population