论文部分内容阅读
目的 探讨线粒体 DNA与人精子活力间的关系。 方法 用长链 PCR技术 ,对 6 0例精子活力正常和 40例精子活力异常不育患者的精子线粒体 DNA(mt DNA)进行了多重缺失的分析。 结果 两组不育患者中共有 8例具有 m t DNA的多重缺失 (其中精子活力正常不育患者 6名 ,精子活力异常不育患者 2名 ) ,但缺失型 mt D-NA(S5除外 )在总 mt DNA中所占比例很小 (0 .16 %～ 1.85 % ) ,1例精子活力正常的不育患者 (S5 )具有 2 .6 kb的缺失型 mt DNA,且缺失型 mt DNA占总 m t DNA的 91.0 2 % ,其序列分析发现 mt DNA编码区域大部分缺失。 结论 精子活力与 mt DNA的多重缺失间无相关性。
Objective To investigate the relationship between mitochondrial DNA and human sperm motility. Methods Multiplex deletion of sperm mitochondrial DNA (mt DNA) in 60 patients with normal sperm motility and 40 patients with abnormal sperm motility was analyzed by long-range PCR. Results Eight infertile patients had multiple deletions of mt DNA (6 in normal sperm motility infertility and 2 in patients with abnormal sperm motility), but the deletion mt D-NA (except S5) The proportion of mt DNA was very small (0.16% -1.85%). One infertile patient with normal sperm motility (S5) had a 2.6 kb deletional mt DNA and the deletion mt DNA accounted for the total mt DNA Of 91.0 2%, the sequence analysis found that most of the mt DNA coding region is missing. Conclusion There was no correlation between sperm motility and multiple deletions of mt DNA.