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目的分析深圳地区5岁以下急性腹泻患儿星状病毒感染的流行病学特点。方法采集深圳市2007年8月~2009年2月413例5岁以下的腹泻患儿腹泻急性期粪便标本,采用RT-PCR技术扩增星状病毒的非结构蛋白基因的289个核苷酸片段,对阳性标本的PCR产物经纯化后测序和序列分析,同时对采集病例的流行病学资料进行统计学分析。结果 413份标本检出星状病毒32例,检出率为7.75%(32/413),星状病毒感染患儿年龄为7d～3岁,<2岁的患儿占93.75%。87.5%的星状病毒感染主要集中在10月至次年1月,与轮状病毒感染季节相似。对AstV非结构蛋白基因进行序列测序和分析,结果与北京株(FJ755355)基因的同源性高达98%,与韩国株(AY962550)的同源性达93%。结论星状病毒是深圳地区婴幼儿病毒性腹泻的重要病原之一。
Objective To analyze the epidemiological characteristics of astrovirus in children under 5 years of age with acute diarrhea in Shenzhen. Methods A total of 289 nucleotide fragments of non-structural protein genes of astrovirus were amplified by RT-PCR from 413 cases of diarrhea children under 5 years of age in the acute phase of diarrhea in Shenzhen City from August 2007 to February 2009. The PCR products of the positive samples were purified and sequenced. The epidemiological data of the collected samples were also analyzed statistically. Results 413 samples were detected as 32 cases of astrovirus, the detection rate was 7.75% (32/413), astrovirus infection in children aged 7d to 3 years, <2 years old children accounted for 93.75%. 87.5% of astrovirus infections mainly concentrated in October to January of the following year, similar to the rotavirus infection season. Sequence analysis and sequencing of the AstV non-structural protein gene showed that the homology of the gene with Beijing strain (FJ755355) was as high as 98%, and that of the Korean strain (AY962550) was 93%. Conclusion Astrovirus is one of the important pathogens of infant diarrhea in Shenzhen.