论文部分内容阅读
目的评价血管紧张素转换酶抑制剂贝那普利及血管紧张素受体拮抗剂缬沙坦对自发性高血压大鼠(SHR)血清性激素、性腺指数及勃起功能的影响。方法将24只11周龄的雄性SHR随机分为3组:缬沙坦组[缬沙坦30mg/(kg·d)溶于0.5mL双蒸水灌胃,A组,n=8],贝那普利组[贝那普利10mg/(kg·d)溶于0.5mL双蒸水灌胃,B组,n=8],SHR对照组(双蒸水0.5mL/d灌胃,C组,n=8)。另设雄性WKY组(双蒸水0.5mL/d灌胃,D组,n=8)。适应环境1周后,测量大鼠初始体质量、收缩压及血清睾酮、血清雌二醇水平;2月后再次测量体质量及收缩压,并通过多道生理信号采集及处理系统测量0、2、5V电刺激时的阴茎海绵体内压(ICP)及平均动脉压(MAP),评估勃起功能(ICP/MAP)。颈动脉收集血样本后处死大鼠,迅速摘取睾丸称重并计算性腺指数(性腺质量/体质量×100),并通过电化学发光法测量血清睾酮、血清雌二醇水平。结果治疗2月后,缬沙坦及贝那普利均降低SHR血压,而对血清睾酮、血清雌二醇水平无明显影响。与A、B、D组比较,C组血清睾酮[(32.39±6.23)比(43.27±9.24)、(41.71±6.81)、(39.08±8.48)nmol/L,P<0.05]降低。与B、D组比较,C组血清雌二醇[(39.14±3.89)比(35.37±1.71)、(33.04±3.98)ng/L,P<0.05]升高。在0、2、5V电刺激海绵体神经时,C组的ICP/MAP最低,D组最高。单因素直线相关分析显示,收缩压、雌二醇与睾酮比值均与ICP/MAP呈明显负相关。各组间睾丸质量与性腺指数在比较,差异均无统计学意义(P>0.05)。结论缬沙坦及贝那普利降低血压的同时不影响血清睾酮、血清雌二醇水平,对SHR勃起功能无损害。
Objective To evaluate the effects of angiotensin converting enzyme inhibitor benazepril and angiotensin Ⅱ receptor antagonist valsartan on serum sex hormones, gonadal index and erectile function in spontaneously hypertensive rats (SHR). Methods Twenty-four male SHRs of 11 weeks old were randomly divided into 3 groups: valsartan group (valsartan 30mg / (kg · d) dissolved in 0.5mL double distilled water, group A, n = 8] The patients in the natriuretic group (benazepril 10mg / (kg · d) dissolved in 0.5mL double distilled water, group B, n = 8], SHR control group , n = 8). Another male WKY group (double distilled water 0.5mL / d gavage, D group, n = 8). After 1 week of adaptation, the initial body weight, systolic blood pressure, serum testosterone and serum estradiol level were measured. Body mass and systolic blood pressure were measured again after 2 months and measured by multi-channel physiological signal acquisition and processing system 0,2 , Intracavernous pressure (ICP) and mean arterial pressure (MAP) at 5 V electrical stimulation, and erectile function (ICP / MAP) were evaluated. The blood samples were collected from the carotid artery and sacrificed. The testes were quickly weighed and the gonadal index (gonadal mass / body weight × 100) was calculated. The levels of serum testosterone and serum estradiol were measured by electrochemiluminescence. Results After 2 months of treatment, both valsartan and benazepril lowered SHR blood pressure, but had no significant effect on serum testosterone and serum estradiol levels. Compared with group A, group B and group D, serum testosterone in group C was significantly lower than that in group C ([(32.39 ± 6.23) vs (43.27 ± 9.24), (41.71 ± 6.81) and (39.08 ± 8.48) nmol / L, respectively; Compared with group B and D, serum estradiol in group C was significantly higher than that in group C (39.14 ± 3.89 vs 35.37 ± 1.71, 33.04 ± 3.98 ng / L, P <0.05). At 0,2,5V electrical stimulation of cavernosal nerve, the ICP / MAP group C the lowest, the highest group D. Single factor linear correlation analysis showed that systolic blood pressure, estradiol and testosterone ratio were significantly negative correlation with ICP / MAP. There was no significant difference in testicular mass and gonadal index between groups (P> 0.05). Conclusion Valsartan and benazepril lower blood pressure without affecting serum testosterone and serum estradiol levels, and have no effect on erectile function of SHR.