为了将目的抗原靶向免疫源发器官,作者将两种分子L-选择素和细胞毒性T细胞抗原4(CTLA4)的胞外结构域基因与目的基因人IgG(hIgG)恒定区连结,建立融合表达载体。已知广泛分布于白细胞表面的L-选择素通过结合高内皮静脉细胞上的CD34,介导白细胞归巢入外周淋巴结;而表达于激活T细胞上的CTLA4与APC表面B7结合,可强烈诱生免疫应答。因此设想通过L-选择素或CTLA4将目的抗原hIgG导向淋巴结和APC,增强免疫应答。 In order to target the antigen of interest to the organ of immune origin, the authors linked the extracellular domain genes of both molecules L-selectin and cytotoxic T cell antigen 4 (CTLA4) to the hIgG constant region of the gene of interest to establish a fusion Expression vector. It is known that L-selectin, which is widely distributed on the surface of leukocytes, mediates the homing of leukocytes to the peripheral lymph nodes by binding to CD34 on high endothelial vein cells; whereas CTLA4, expressed on activated T cells, binds to B7 on the surface of APCs and can be strongly induced Immune response. Therefore, it is envisaged that the target antigen hIgG is directed to lymph nodes and APCs by L-selectin or CTLA4 to enhance the immune response.