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用随机扩增多态性DNA(RandomamplifiedpolymorphicDNA ,RAPD)技术对来自陕西及青海的 7个地区的 1 3个落叶松—杨栅锈菌 (Melampsoralarici populinaKleb.)的分离物进行了基因组DNA多态性分析。 1 3个 1 0 -核苷酸随机引物 (Operon公司 )对 1 3个菌株共扩增出 81条RAPD带 ,其中 69个DNA片断呈现多态性 ,占总扩增片断的 85.2 %。供试菌株的相似系数在 0 .60 8～ 1 .0 0 0之间 ,各菌株之间的差异在 0～3 3 1 %之间 ,并建立了聚类树状图。 1 3个菌株在相似性 76.1 %时被分为 4个类群 :I组包括秦岭宁陕火地塘C的 2个分离物 ,II组为火地塘B的 1个分离物 ;III组为青海西宁、互助 ,陕西太白宝太路、宝鸡天台山、周至厚畛子 (HZa)等 5个地区的 8个分离物 ;第IV组为陕西周至厚畛子的 2个菌株 (HZb、HZc)。并与各菌株对不同杨树致病性的聚类分析进行了比较 ,结果表明各菌株间的DNA多态性与致病性及寄主、地理来源等没有明显相关性。
The genomic DNA polymorphisms of 13 isolates of Melampsoralarica populina Kleb from seven regions of Shaanxi and Qinghai were analyzed by using random amplified polymorphic DNA (RAPD) . A total of 81 RAPD bands were amplified from 13 isolates by Oeron, a 3 10-nucleotide random primer. Among them, 69 DNA fragments showed polymorphism, accounting for 85.2% of the total amplified fragments. The similarity coefficients of the tested strains ranged from 0.608 to 1.000, and the differences among the strains ranged from 0 to 33.1%, and the cluster dendrogram was established. The three strains were divided into four groups at the similarity of 76.1%: Group I consisted of two isolates from Coticidium of Wutai in Ningxia, Qinling and Group II, one isolate of B in Huoditang; Group III was Qinghai Xining, Mutual Aid, Shaanxi Taibaibao Taolu, Baoji Tiantai Mountain, Zhouzhihouchuzi (HZa) and other five regions of the eight isolates; the fourth group of Shaanxi Zhou Zhi Houzi two strains (HZb, HZc). The results showed that there was no significant correlation between DNA polymorphism and pathogenicity, host and geographical origin of each strain.