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目的:探讨T淋巴瘤侵袭转移诱导因子1(Tiam 1)表达与肾癌发生、发展和转移的关系。方法:应用原位杂交及免疫组化方法检测正常肾组织、肾癌石蜡组织标本中Tiam 1mRNA和蛋白的表达情况。用三种不同浓度2μmol/L(T1低浓度)、4μmol/L(T2中浓度)、8μmol/L(T3高浓度)的索坦干扰786-0细胞24h、48h、72h;同时设正常对照组T4(常规培养液)。应用TUNEL法检测各组作用48h细胞凋亡率的变化。应用原位杂交及免疫组化方法检测各组Tiam 1mRNA和蛋白的表达情况。结果:68例肾细胞癌组织中Tiam 1阳性表达率63.2%(43/68),有淋巴结转移组Tiam 1表达率明显高于无淋巴结转移组(P<0.05),TNM分期为Ⅲ～Ⅳ期高于Ⅰ～Ⅱ期,差异有统计学意义(P<0.01);对照组10例正常肾组织中Tiam 1表达均为阴性。Tiam 1基因在786-0细株中表达与肾癌组织中表达相一致。结论:Tiam 1表达与肾癌转移存在密切关系,Tiam 1表达可作为肾癌转移过程中一个有价值的指标;索坦可抑制肾癌细胞的生长,诱导细胞的凋亡,可特异性抑制肾癌786-0细胞中Tiam 1蛋白及mRNA的表达,且具有浓度和时间依赖性。
Objective: To investigate the relationship between the expression of Tiam 1 in T lymphoma and the occurrence, development and metastasis of renal cell carcinoma. Methods: In situ hybridization and immunohistochemistry were used to detect the expression of Tiam 1 mRNA and protein in normal renal tissue and renal cell carcinoma. 786-0 cells were treated with three concentrations of 2μmol / L (T1 low concentration), 4μmol / L (T2 medium concentration) and 8μmol / L (T3 high concentration) for 24 hours, 48 hours and 72 hours respectively. At the same time, T4 (normal medium). TUNEL method was used to detect the changes of apoptotic rate in each group at 48h. In situ hybridization and immunohistochemistry were used to detect the expression of Tiam 1 mRNA and protein in each group. Results: The positive rate of Tiam 1 in 68 cases of renal cell carcinoma was 63.2% (43/68), the expression of Tiam 1 in lymph node metastasis was significantly higher than that in non-lymph node metastasis (P <0.05) Higher than Ⅰ ~ Ⅱ stage, the difference was statistically significant (P <0.01); control group of 10 cases of normal renal tissue expression of Tiam 1 were negative. The expression of Tiam 1 gene in 786-0 strain is consistent with the expression in renal carcinoma. Conclusion: The expression of Tiam 1 is closely related to the metastasis of renal cell carcinoma. The expression of Tiam 1 can be used as a valuable marker in the metastasis of renal cell carcinoma. Sotalol can inhibit the growth of renal carcinoma cells, induce cell apoptosis and specifically inhibit renal Tiam 1 protein and mRNA expression in cancer 786-0 cells in a concentration and time dependent manner.