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目的:观察强肌健力饮对脾虚大鼠血清T3(三碘甲腺原氨酸)、T4(甲状腺素)、rT3(3,3′,5′-三碘甲腺原氨酸)、TSH(促甲状腺激素)含量的影响,以期进一步了解该方药对脾虚证的药理作用,为临床用药提供实验依据。方法:选用SD雄性大鼠,随机分为正常对照组(n=10)、脾虚模型组(n=12)、强肌低剂组(n=13)、强肌中剂组(n=13)、强肌高剂组(n=11)、四君子汤组(n=10)。采用大黄复制脾虚模型,分别于上午给予蒸馏水、大黄水煎剂灌胃;下午分别灌以等量的蒸馏水及相应剂量的强肌健力饮和四君子汤,连续用药20天,实验结束后取大鼠血清,观察各组大鼠甲状腺激素的含量变化。结果:与正常对照组比较,脾虚模型组血清T3、T4分泌减少,有显著性差异(P<0.01);rT3分泌有所增加但尚未统计学差异,TSH无反馈性升高(P>0.05)。强肌健力饮高剂量使下降的T3、T4含量均明显升高,有显著性差异(P<0.01),强肌健力饮低剂量在恢复T4方面有明显作用(P<0.01)。四君子汤对脾虚引起的T3、T4分泌减少亦有明显的恢复作用(P<0.01)。结论:强肌健力饮对脾虚证大鼠有一定的保护作用,其作用机理之一与调节甲状腺激素水平有关。
Objective: To observe the effects of Qiangji Jianli Yin on serum T3 (triiodothyronine), T4 (thyroxine), rT3 (3,3 ’, 5’-triiodothyronine), TSH (Thyroid stimulating hormone) content, in order to further understand the pharmacological effects of the prescription on spleen deficiency syndrome, providing experimental evidence for clinical use. Methods: SD male rats were randomly divided into normal control group (n = 10), spleen deficiency model group (n = 12), low muscle strong muscle group (n = 13) , Strong muscle high agent group (n = 11), Sijunzi Decoction group (n = 10). Rhubarb copy spleen deficiency model, respectively, in the morning to give distilled water, rhubarb water decoction gavage; afternoon were dosed with the same amount of distilled water and the corresponding dose of strong muscle Jianli Yin and Sijunzi Decoction, continuous medication for 20 days after the experiment to take large Rat serum to observe the changes of thyroid hormones in each group. Results: Compared with the normal control group, the secretion of T3 and T4 in the spleen deficiency model group decreased significantly (P <0.01); the secretion of rT3 was increased but not yet statistically significant (P> 0.05) . Qiangjian Jianlian decoction high dose decreased T3, T4 content were significantly increased, there was a significant difference (P <0.01), low muscle strong Jianlian low dose in the recovery of T4 has a significant effect (P <0.01). Sijunzitang spleen deficiency caused by T3, T4 secretion also had a significant recovery (P <0.01). Conclusion: Qiangji Jianli Decoction can protect rats with spleen deficiency syndrome. One of its mechanisms is related to the regulation of thyroid hormones.