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在加与不加Ｓ９两种情况下，用Ｖ７９中国仓鼠细胞检测了Ｂ（α）Ｐ诱导的ＨＧＰＲＴ突变频率和细胞毒性。结果表明，无论加与不加Ｓ９混合液，Ｂ（α）Ｐ对Ｖ７９细胞都具有明显毒作用，但在不加Ｓ９时，不会导致６—巯基鸟嘌呤抗性细胞发生率的明显增加．在存在Ｓ９混合液时，Ｂ（α）Ｐ诱导的ＨＧＰＲＴ突变频率随Ｂ（α）Ｐ浓度的加大而明显增加。剂量在０．５—８μｇ／ｍｌ范围时，每１μｇ／ｍｌＢ（α）Ｐ诱发的Ｖ７９细胞ＨＧＰＲＴ位点突变频率为７—２４／１０６细胞。
The B(α)P-induced HGPRT mutation frequency and cytotoxicity were detected in V79 Chinese hamster cells with and without addition of S9. The results showed that B(α)P had obvious toxic effects on V79 cells with or without S9 mixture, but it did not lead to a significant increase in the incidence of 6-thioguanine-resistant cells when S9 was not added. In the presence of S9 mixture, the frequency of HGPRT mutation induced by B(α)P increased significantly with the increase of B(α)P concentration. When the dose was in the range of 0.5-8 μg/ml, the HGPRT site mutation frequency was 7-24/106 cells per 1 μg/ml B(α)P-induced V79 cells.