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新型杀虫蛋白基因cry1Ah基因是中国农业科学院植物保护研究所从Bt菌株BT8中鉴定克隆的,其编码蛋白对鳞翅目害虫具有强毒力,尤其对亚洲玉米螟Ostrinia furnacalis(Guenée)的毒力强于目前使用的cry1A类基因。转cry1Ah基因抗虫玉米具有很好的应用前景。花粉是蜜蜂重要的食物来源,蜜蜂是转基因植物安全性评价的关键测试生物。因此,开展转cry1Ah基因玉米对蜜蜂的安全性研究很有必要。给意大利蜜蜂Apis mellifera ligustica Spinola蜂群中4～6日龄幼虫饲喂转基因玉米花粉、常规玉米花粉、杂花粉,哺育蜂饲喂为对照。转基因玉米花粉对意大利蜜蜂封盖率、出房率和发育历期没有显著影响。表明转cry1Ah基因玉米花粉对意大利蜜蜂幼虫的存活和发育没有不良影响。
The cry1Ah gene of the new insecticidal protein gene was identified from the Bt strain BT8 by the Institute of Plant Protection, Chinese Academy of Agricultural Sciences. The encoded protein has strong virulence against lepidopteran pests, especially against Ostrinia furnacalis (Guenée) Stronger than the cry1A gene currently used. Turn cry1Ah insect-resistant corn has a good application prospects. Pollen is an important source of food for bees, and bees are key test organisms for the safety evaluation of transgenic plants. Therefore, it is necessary to carry out the research on the safety of bee transformed with cry1Ah maize. Four to six-day-old larvae of Apis mellifera ligustica Spinola were fed with maize pollen, conventional corn pollen, hybrid pollen and nursery feed for the control. Transgenic corn pollen had no significant effect on the beet seal rate, atrial outflow rate and developmental duration in Italy. The results showed that transgenic poll with cry1Ah gene had no adverse effect on the survival and development of Italian bee larvae.