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利用POPGENE 3.2软件对13个不同来源的大麻群体进行遗传多样性分析。结果显示:云南地区的大麻群体具有最高的遗传多样性水平(PPB=88.82%,He=0.3000,I=0.4571),其次为黑龙江群体(PPB=75.66%,He=0.2572,I=0.3897)。13个大麻群体的多态位点百分率(PPB)为92.11%,Nei’s总遗传多样性(Ht)为0.3837,Shannon’s信息指数I=0.5374。群体内遗传多样性(Hs)为0.1640,群体间的遗传分化系数(Gst)为0.5725,总的遗传变异中有57.25%发生在群体间,42.75%发生在群体内。根据Nei’s(1978)的方法计算了13个大麻群体间的遗传距离和遗传一致度。结果显示:各群体间的遗传一致度在0.6556～0.9258之间,其中四川群体和广西群体间具有最高的遗传一致度(0.9258);云南群体与贵州群体和四川群体间遗传一致度分别为0.9196、0.9173。所有群体中甘肃群体和山西群体遗传一致度最低为0.6556,说明大麻种内具有较大的遗传变异。
Genetic diversity analysis was conducted on 13 cannabis populations from different sources using POPGENE 3.2 software. The results showed that the highest genetic diversity of cannabis was found in Yunnan (PPB = 88.82%, He = 0.3000, I = 0.4571), followed by Heilongjiang (PPB = 75.66%, He = 0.2572, I = 0.3897). The percentage of polymorphic loci (PPB) of 13 cannabis populations was 92.11%, Nei’s total genetic diversity (Ht) was 0.3837, Shannon’s information index I = 0.5374. The genetic diversity (Hs) was 0.1640 in the population, the genetic differentiation coefficient (Gst) was 0.5725 in the population, 57.25% of the total genetic variation occurred in the population, and 42.75% occurred in the population. The genetic distance and genetic identity of 13 cannabis populations were calculated according to Nei’s (1978). The results showed that the genetic identity of each population was between 0.6556 and 0.9258, with the highest genetic identity (0.9258) between Sichuan and Guangxi populations; the genetic identity between Yunnan and Guizhou and Sichuan was 0.9196, 0.9173. The genetic identity of Gansu and Shanxi populations in all the groups was the lowest, which was 0.6556, which indicated that there was a large genetic variation in cannabis species.