论文部分内容阅读
利用在玉米中克隆到的一个抗水稻细菌性条斑病的非寄主抗性基因Rxo1中含有NBS-LRR结构的开放读码框(ORF)在水稻基因组中搜索到16个抗性基因同源序列,进一步研究发现,位于第11染色体上的同源序列基因RPR1与细菌性条斑病的抗性有一定关系。首先,以RPR1为模板设计的两对引物都只在供试的细菌性条斑病抗病品种中扩增出目标带;再者RT-PCR的结果表明RPR1的表达能被细菌性条斑病菌接种所诱导,说明来自不同物种的结构相似的抗性基因可以表达相同或相似的功能。但从对RPR1定位的结果看,RPR1与之前定位的抗细菌性条斑病主效QTL之间尚有9cM的图距,两者之间的连锁并不紧密,说明RPR1的表达并不能解释抗病品种Dular对细菌性条斑病的抗性,RPR1并不是水稻表达细菌性条斑病抗性的关键因子。
In the present study, 16 open reading frames (ORFs) containing the NBS-LRR structure in the non-host resistance gene Rxo1 of rice bacterial leaf spot cloned in maize were used to search for 16 resistance gene homologous sequences Further study found that RPR1, a homologous gene located on chromosome 11, has some relationship with the resistance of bacterial leaf streak. First of all, two pairs of primers designed with the template of RPR1 only amplified the target band in the tested bacterial leaf stalk resistant varieties; furthermore, the results of RT-PCR indicated that the expression of RPR1 could be contaminated by Bacterial leaf spot bacteria Inoculation induced, indicating that structurally similar resistance genes from different species may express the same or similar functions. However, from the results of RPR1 mapping, there is still a 9cM distance between the RPR1 locus and the main QTL for antibacterial streak blight. The linkage between RPR1 and RPR1 is not very close, indicating that the expression of RPR1 does not explain the resistance Resistance of the disease variety Dular to bacterial leaf streak, RPR1, is not a key factor in rice resistance to bacterial leaf streak.