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本文初步分离纯化了人尿中的内生致冷原(EC)。利用硫酸铵沉淀、超滤和分子筛凝胶柱层析等技术,筛选出具降温活性的尿液,获得初步分离纯化的EC。经SDS-PAGE鉴定,此EC主要由S5、43、30、25、16KD的蛋白质组成,并在0.4～1.6ml/km范围呈剂量依赖关系。
This article preliminary isolation and purification of endogenous human genistein (EC). Ammonium sulfate precipitation, ultrafiltration and molecular sieve gel column chromatography and other techniques to screen the urine with cooling activity, to obtain the preliminary isolation and purification of EC. The results of SDS-PAGE showed that the EC mainly consisted of S5, 43, 30, 25, and 16 KD proteins and showed a dose-dependent manner in the range of 0.4-1.6 ml / km.