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用扫描电镜观察的样品要求表面能导电,惯用的方法是在样品表面蒸涂导电层,对于表面起伏大而又容易变形的生物样品,这种方法效果不太好。近年来采用组织导电技术处理生物样品取得了比较好的效果,但一般对镜体污染大增加了仪器维护的麻烦。我们使用多种导电液处理样品在S—100电镜上面进行比较,觉得用四氯化锡导电液处理方法简单对镜体污染小。方法如下:把待观察样品按常规固定后保存在70％酒精中,把SnCl_4放入75％的酒精溶液中配制成5％的酒精四氯化锡导电液。把保存在酒精中的样品捞放入导电液中适当浸泡取出胶合即可观察。如果经过乙腈干燥后再
The samples that are observed by scanning electron microscopy (SEM) require the surface energy to conduct electricity. The usual method is to steam-coat the surface of the sample with a conductive layer. This method is not effective for biological samples that have a large surface and are easily deformed. In recent years, the use of tissue conduction technology to deal with biological samples have achieved good results, but the general body of the mirror pollution increased maintenance of the trouble. We use a variety of conductive liquid sample processing in the S-100 electron microscopy above to compare, that the use of tin tetrachloride conductive liquid treatment method is simple on the mirror body pollution is small. The method is as follows: the sample to be observed is routinely stored in 70% alcohol and SnCl_4 in 75% alcohol solution to prepare a 5% alcohol solution of tin tetrachloride. The sample stored in alcohol fishing into the conductive liquid in the appropriate immersion remove the glue can be observed. If after after drying acetonitrile