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本研究旨在探讨育龄妇女输卵管中钙结合蛋白calbindin-D28k(CaBP-D28k)的表达和变化。33例月经规律、有正常生育史的育龄妇女,因子宫肌瘤、子宫腺肌症行子宫切除术(一并切除输卵管),输卵管取材均包括峡部、壶腹部和伞部,按月经周期分为增生早期组6例,增生中期组5例、增生晚期组5例,分泌早期组7例,分泌中期组5例和分泌晚期组5例。采用免疫组织化学法和逆转录聚合酶链反应法检测CaBP-D28k在人输卵管组织中的表达。结果显示,人输卵管组织中存在CaBP-D28k蛋白及mRNA表达。CaBP-D28k蛋白表达于输卵管上皮细胞的胞浆中,在月经周期同一时期,输卵管峡部、壶腹部、伞部的表达无明显差别(P>0.05)。在月经周期中,CaBP-D28k蛋白表达在增生早、中期最低,而在增生晚期和分泌早期明显增高(P<0.05),分泌中、晚期显著下降(P<0.05)。CaBP-D28k蛋白阳性表达分布在月经周期中出现再分布,在增生早、中期呈灶状或片状弥散分布在胞浆中,增生晚期和分泌早、中期CaBP-D28k蛋白呈聚集的颗粒状集中在细胞顶部,至分泌晚期则仍呈灶状或弥散状分布;CaBP-D28kmRNA表达也随月经周期发生变化,在增生晚期和分泌早期明显增高(P<0.05)。结果表明,人输卵管组织中存在CaBP-D28k蛋白及mRNA表达,且具有周期性变化。
The purpose of this study was to investigate the expression and alteration of calbindin-D28k (CaBP-D28k) in fallopian tubes in women of childbearing age. 33 cases of menstrual regularity, women of childbearing age with normal fertility history, due to uterine fibroids, adenomyosis hysterectomy (together with the removal of the fallopian tubes), fallopian tubes are drawn from the isthmus, ampulla and umbrella, according to the menstrual cycle is divided into 6 cases of early proliferative group, 5 cases of middle proliferative group, 5 cases of late proliferative group, 7 cases of early secretory group, 5 cases of secretory middle stage and 5 cases of advanced secretory stage. The expression of CaBP-D28k in human oviduct was detected by immunohistochemistry and reverse transcription polymerase chain reaction. The results showed that human oviduct tissue CaBP-D28k protein and mRNA expression. CaBP-D28k protein expression in the tubal epithelial cells in the cytoplasm, in the same period of the menstrual cycle, tubal isthmus, ampulla, umbrella no significant difference in the expression (P> 0.05). In the menstrual cycle, CaBP-D28k protein expression in the early and mid-term proliferation of the lowest, but in the early and early stages of proliferation significantly increased (P <0.05), the secretion of late, significantly decreased (P <0.05). CaBP-D28k protein positive distribution in the menstrual cycle redistribution, in the early and mid-proliferative focal or lamellar diffuse distribution in the cytoplasm, late proliferative and early, middle CaBP-D28k protein aggregated particle concentration At the top of the cells, the distribution of CaBP-D28kmRNA was still focal or diffuse till the late stage of secretion. The expression of CaBP-D28kmRNA also changed with the menstrual cycle, and was significantly increased in the late stage of hyperplasia and the early stage of secretion (P <0.05). The results showed that human tubal tissue exists CaBP-D28k protein and mRNA expression, and with periodic changes.