论文部分内容阅读
目的探讨铜绿假单胞菌注射液在体外对胃癌细胞杀伤作用,并观察其在进展期胃癌围手术期应用对预防术后腹腔复发与转移的作用。方法2005年1月至2007年12月上海交通大学医学院附属仁济医院普外科采用四甲基偶氮唑盐(MTT)法观察铜绿假单胞菌注射液抑制胃癌瘤株MKN45细胞增殖和杀伤作用的剂量依赖性;采用扫描电镜观察对胃癌瘤株MKN45的特异性结合。在此基础上选择T3M0进展期胃癌病人共60例,随机分为治疗组和对照组,观察其不良反应和对病人免疫学指标、术后腹腔复发及转移的影响。结果铜绿假单胞菌注射液能与胃癌瘤株MKN45细胞发生特异性结合并可进入细胞内抑制其增殖和生长,其杀伤作用随着用药浓度增加而加强。其安全性较好,用药组病人细胞免疫功能明显增强,腹膜复发和腹水脱落癌细胞阳性率显著低于对照组。结论铜绿假单胞菌注射液有直接杀伤胃癌瘤株MKN45细胞的作用,且这种作用存在剂量依赖性;作为腹腔免疫化疗重要手段,可以增强机体细胞免疫功能、有效预防进展期胃癌术后近期腹腔复发和转移。
Objective To investigate the killing effect of Pseudomonas aeruginosa injection on gastric cancer cells in vitro and to observe the effect of perioperative application of Pseudomonas aeruginosa injection in prevention and treatment of postoperative abdominal cavity recurrence and metastasis. Methods January 2005 to December 2007 Department of General Surgery, Renji Hospital Affiliated to Shanghai Jiaotong University School of Medicine Adopted tetramethoxysilyl salt (MTT) method to observe the proliferation and killing of gastric cancer cell line MKN45 by Pseudomonas aeruginosa injection Dose-dependent. The specific binding of gastric cancer cell line MKN45 was observed by scanning electron microscopy. On this basis, a total of 60 patients with T3M0 advanced gastric cancer were selected and randomly divided into treatment group and control group. The adverse reactions, immunological parameters and postoperative abdominal recurrence and metastasis were observed. Results Pseudomonas aeruginosa injection could specifically bind to gastric cancer cell line MKN45 and could enter the cell to inhibit its proliferation and growth. The killing effect of Pseudomonas aeruginosa was enhanced with the increase of drug concentration. The safety is better. The immune function of the patients in the medication group is obviously enhanced. The positive rate of peritoneal recurrence and ascites drop cancer cells is significantly lower than that of the control group. Conclusion Pseudomonas aeruginosa injection has the effect of directly killing gastric cancer cell line MKN45, and this effect exists in a dose-dependent manner. As an important method of intraperitoneal immunotherapy, it can enhance the cellular immune function and effectively prevent the progression of advanced gastric cancer Abdominal recurrence and metastasis.