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目的建立抗肿瘤转基因小鼠模型。方法用ＢａｍＨＩ酶切表达质粒ｐＵＬＢ３２３８获取小鼠细小病毒非结构基因，通过显微注射法将其接种入小鼠受精卵内制备转基因小鼠。转基因鼠尾部组织ＤＮＡ以ＰＣＲ检测靶基因整合；整合转基因的Ｇ０Ａ６小鼠与正常Ｃ５７ＢＬ／６Ｊ小鼠配种所产Ｇ１代转基因鼠，通过ＲＴ┐ＰＣＲ检测目的基因在其肺脏等组织的转录；Ｇ１代鼠根据ＰＣＲ检测结果分整合和未整合非结构基因两组，两组均腹腔注射致肺肿瘤化学致癌剂氨基甲酸乙酯水溶液。结果Ｇ０代有４只鼠整合靶基因；Ｇ１代（Ｇ０Ａ６子代）转基因鼠的肺组织有靶基因转录；Ｇ１代整合转基因实验组小鼠被诱导肺瘤结节平均数显著少于未整合转基因的对照组（Ｐ＜０．０１）。结论抗肺肿瘤转基因小鼠模型已建立。
Objective To establish an anti-tumor transgenic mouse model. Methods The recombinant plasmid pULB3238 was digested with BamHI to obtain the mouse parvovirus nonstructural gene. The recombinant plasmid was inoculated into the zygotes of mice to prepare transgenic mice by microinjection. Transgenic mouse tail tissue DNA was detected by PCR for target gene integration; G0A6 transgenic mice were inoculated with normal C57BL / 6J mice to produce G1 transgenic mice, RT PCR was used to detect the transcription of the target gene in its lung tissues; Rats according to PCR test results were divided into two groups of non-structural gene integration and integration, two groups were intraperitoneal injection of lung cancer chemical carcinogen urethane aqueous solution. Results There were 4 mice in G0 generation with integrated target genes. The G1 generation (G0A6 offspring) transgenic mice had target gene transcription in the lung tissue. The mice in the G1 transgenic integration group were significantly less in average than the unincorporated transgenes Control group (P <0.01). Conclusion Anti-lung tumor transgenic mouse model has been established.