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目的探讨p16蛋白表达与p16基因突变在鼻咽癌发生中的作用,以确定两者之间的联系及意义。方法运用免疫组化二步法检测鼻咽癌中p16蛋白表达状况;采用多重PCR法检测鼻咽癌中p16基因第二外显子可能的缺失突变。结果p16蛋白表达缺失率:鼻咽癌组中55.3%(26/47),慢性鼻咽炎8%(2/25),鼻咽癌组中p16蛋白表达缺失率高于慢性鼻咽炎组(P<0.001);p16基因第二外显子缺失突变:鼻咽癌组中纯合缺失突变率34%,而相应的癌旁组织未检出(P<0.001);鼻咽癌p16蛋白表达缺失率高于p16基因外显子2缺失率。结论鼻咽癌的发生不仅与p16基因表达缺失和突变密切相关,而且p16蛋白表达下调可能是鼻咽癌发生发展的主要原因。
Objective To investigate the role of p16 protein and p16 gene mutation in the pathogenesis of nasopharyngeal carcinoma in order to determine the relationship between the two and its significance. Methods The expression of p16 protein in nasopharyngeal carcinoma was detected by immunohistochemical two-step method. The possible deletion mutation of exon 2 of p16 gene in nasopharyngeal carcinoma was detected by multiplex PCR. Results The deletion rate of p16 protein in nasopharyngeal carcinoma was 55.3% (26/47) and 8% (2/25) in chronic nasopharyngitis. The loss of p16 protein in nasopharyngeal carcinoma was higher than that in chronic nasopharyngitis (P < 0.001). The second exon deletion mutation of p16 gene: the rate of homozygous deletion mutation in nasopharyngeal carcinoma was 34%, while the corresponding paracancerous tissue was not detected (P <0.001). The loss of p16 protein in nasopharyngeal carcinoma was high Exon 2 deletion rate in p16 gene. Conclusion The occurrence of nasopharyngeal carcinoma is not only closely related to the deletion and mutation of p16 gene, but also the down-regulation of p16 protein may be the main reason for the occurrence and development of nasopharyngeal carcinoma.