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目的调查人群中人疱疹病毒(HHV),包括人巨细胞病毒(HCMV)、EB病毒(EBV)、HHV-6和HHV-7的感染情况。方法收集55例南京市无偿献血者的外周血标本,提取全血DNA,巢氏PCR扩增HCMV、EBV、HHV-6和HHV-7特异性序列,1%琼脂糖凝胶电泳鉴定。结果 55例献血者中EBV、HCMV、HHV-6和HHV-7的阳性感染率分别为50.9%、5.5%、49.1%和21.8%,并且存在多种病毒混合感染。结论 HHV筛查对临床相关疾病的诊治具有十分重要的意义,应引起医务工作者的重视。
Objective To investigate the prevalence of human herpesvirus (HHV), including human cytomegalovirus (HCMV), Epstein-Barr virus (EBV), HHV-6 and HHV-7. Methods Peripheral blood samples from 55 blood donors in Nanjing were collected and whole blood DNA was extracted. The specific sequences of HCMV, EBV, HHV-6 and HHV-7 were amplified by nested PCR and identified by 1% agarose gel electrophoresis. Results The positive rates of EBV, HCMV, HHV-6 and HHV-7 in 55 blood donors were 50.9%, 5.5%, 49.1% and 21.8% respectively, and there were multiple mixed infections. Conclusion HHV screening is very important for the diagnosis and treatment of clinically relevant diseases, which should be paid more attention to by medical workers.