目的探索经济、稳定的肝星状细胞提取和培养方法。方法采用胶原酶改良原位循环灌流、18%Nycodenz密度梯度离心法分离大鼠肝星状细胞,胎牛血清DMEM培养星状细胞;desmin、αSMA和ICAM1免疫细胞化学检测进行星状细胞性质鉴定。结果肝星状细胞的得率稳定在3.5×107/肝以上,纯度为96%～98%左右,存活率为96%～98%。结论建立了经济、稳定的大鼠肝星状细胞提取和培养方法;传代培养可获得较为稳定的细胞系。 Objective To explore an economical and stable method for the extraction and culture of hepatic stellate cells. Methods The rat hepatic stellate cells were isolated by 18% Nycodenz density gradient centrifugation and the stellate cells were cultured with fetal calf serum (DMEM). Collagenase was used to improve the activity of stellate cells. Desmin, αSMA and ICAM1 were used to detect the characteristics of stellate cells. Results The yield of hepatic stellate cells stabilized at 3.5 × 107 / liver, with a purity of 96% -98% and a survival rate of 96% -98%. Conclusion The establishment of an economical and stable method for the extraction and culture of rat hepatic stellate cells, and the more stable cell lines can be obtained by subculturing.