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用大白鼠建立高脂模型,喂食不同剂量的大花葵花色苷(0.03 g-d-1, 0.04 g-d-1, 0.05 g-d-1)。结果表明:0.04 g-d-1 花色苷剂量可以使大白鼠总胆固醇下降19.7%,0.05 g-d-1的花色苷剂量可以使甘油三酯下降34.4%。通过体外试验,用邻二氮菲Fe2+氧化还原法进行自由基清除能力的测定,抗脂质过氧化的测定。发现当大花葵花色苷浓度为0.2 mg-mL-1时,对自由基的清除率可达43.46%;当浓度为0.5 mg-mL-1时,对脂质氧化的抑制率达18.82%。由此看出,花色苷是一种天然有效的自由基清除剂和抗氧化剂,可预防血栓的形成,有助于减少心脑血管疾病的发生。表3 图5 参16。
Rats were established with a high-fat model and fed different doses of Saffron anthocyanins (0.03 g-d-1, 0.04 g-d-1, 0.05 g-d-1). The results showed that the dose of 0.04 g-d-1 anthocyanin could reduce the total cholesterol of rats by 19.7%, and the anthocyanin dose of 0.05 g-d-1 could decrease the triglyceride by 34.4%. The in vitro test was performed to determine the free radical scavenging ability and the anti-lipid peroxidation assay using ortho-phenanthroline Fe2+ redox method. When the concentration of anthocyanin was 0.2 mg-mL-1, the free radical scavenging rate was 43.46%. When the concentration was 0.5 mg-mL-1, the inhibitory rate of lipid oxidation was 18.82%. It can be seen that anthocyanins are natural and effective free radical scavengers and antioxidants, which can prevent the formation of thrombus and help reduce the occurrence of cardiovascular and cerebrovascular diseases. Table 3 Figure 5 Reference 16.