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目的:探讨三七总皂甙对微循环障碍的主要白细胞与血管内皮的粘附因子的抑制作用。方法:采用内毒素(LPS)连续静脉给药的方法建立大鼠肠系膜微循环障碍模型,观察三七总皂甙对白细胞及静脉内皮粘附的抑制作用,并用流式细胞仪检测外周血粒细胞粘附因子CD11b、CD18的表达。结果:在LPS静脉滴注20min后,静脉血管壁的白细胞数显著增多,三七总皂甙可以显著抑制白细胞及内皮细胞的粘附,在体外LPS刺激可显著增加中性粒细胞表面粘附因子的表达,而三七总皂甙可以抑制粒细胞CD11b和CD18的表达,差异具显著性。结论:三七总皂甙通过抑制细胞粘附因子CD11b及CD18的表达,起到抑制白细胞血管壁粘附的作用。
Objective: To investigate the inhibitory effect of Panax notoginseng saponins on adhesion factors of major white blood cells and vascular endothelium in patients with microcirculatory disturbance. METHODS: A rat model of mesenteric microcirculation was established by continuous intravenous administration of endotoxin (LPS). The inhibitory effect of Panax notoginseng saponins on leukocyte and vein endothelium adhesion was observed. Peripheral blood granulocyte adhesion was measured by flow cytometry. Annexin CD11b, CD18 expression. RESULTS: After 20 minutes of intravenous infusion of LPS, the number of leukocytes in the venous vessel wall increased significantly. Panax notoginseng could significantly inhibit the adhesion of leukocytes and endothelial cells. In vitro LPS stimulation significantly increased the adhesion factor of neutrophils. The expression of Panax Notoginseng could inhibit the expression of CD11b and CD18 in granulocytes, and the difference was significant. Conclusion: Panax Notoginseng Saponins can inhibit the adhesion of leukocyte vascular wall by inhibiting the expression of CD11b and CD18.