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目的在重组人骨形态发生蛋白-2(rhBMP-2)诱导异位成骨过程中,局部应用不同浓度的辛伐他汀(SIM),探讨SIM对rhBMP-2诱导成骨的作用。方法 SD大鼠分3组。A组:高剂量实验组;B组:低剂量实验组;C组:对照组。将复合体植入大鼠股后部肌袋中,术后15d、30d、60d对实验大鼠进行大体观察、生化检测。结果大体观察:各组组织块均随着时间延长而变硬,范围变大。A、B、C三组组织标本AKP含量均于术后30d达到峰值。第15天A组、B组AKP含量显著低于C组AKP含量;第30天,A组AKP含量明显低于C组和B组;B、C两组间则无明显差别;第60天,各组间AKP含量均无明显差别,且含量均较低。结论辛伐他汀与rhBMP-2之间在促进异位成骨方面不具有协同作用;高浓度辛伐他汀可明显抑制rhBMP-2的促进成骨作用。
Objective To investigate the effect of simvastatin on osteogenic differentiation induced by rhBMP-2 induced by recombinant human bone morphogenetic protein-2 (rhBMP-2) in different levels of simvastatin (SIM) during the process of ectopic osteogenesis. Methods SD rats were divided into 3 groups. Group A: high-dose experimental group; Group B: low-dose experimental group; Group C: control group. The composite was implanted into the posterior muscle of rats and the rats were observed and biochemically detected 15d, 30d and 60d after operation. The results of the general observation: each group of tissue blocks harden with time, the range becomes larger. A, B, C three groups of tissue samples AKP content peaked 30d after surgery. AKP content in group A and group B was significantly lower than that in group C on day 15. On day 30, AKP content in group A was significantly lower than those in group C and group B. There was no significant difference between group B and C on day 30. On day 60, AKP content between the groups showed no significant difference, and the content is low. Conclusion Simvastatin and rhBMP-2 have no synergistic effect in promoting ectopic osteogenesis. Simvastatin at high concentration can significantly inhibit the osteogenesis of rhBMP-2.