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用SDS梯度聚丙烯酰胺凝胶电泳分析了Glycine亚属10个种共26份收集物的种子蛋白,片与soja亚属的典型种子蛋白谱进行比较。一般说来,多年生种含有与soja亚属的大豆球蛋白和β—副大豆球蛋白相似的相应迁移率的种子蛋白,但变异更为广泛,除β—副大豆球蛋白各亚基定位的凝胶区域里变异更显著而普遍外,还观察到大豆球蛋白A、B亚基定位的凝胶区域中电泳带型的变异。每种都产生一个独特的电泳谱而能相互区别。根据种子蛋白电泳类型,9个多年生种可分为三组。第一定组仅G.tomentella一种,其电泳谱与soja亚属最为接近。第二组包括G.cladestina、G.canescens G.latrobeana和G.argyraea四种,第三组由G.tabacina、G.latifolia、G.cyrtola6a和G.falcata四种组成。而G.wightii的电泳谱是最为独特的。在多数种内各收集物之间观察到相对小的种子蛋白变异。
A total of 26 collections of 10 subgenuses of Glycine genus were analyzed by SDS gradient polyacrylamide gel electrophoresis to compare their protein profiles with the typical seed protein profiles of subgenus soja. In general, perennial species contain seed proteins with similar mobilities similar to those of the subgenus soja and β-paramylon globulin, but the variation is more extensive, with the exception of the condensate localized to each subunit of β-paramylon globulin Variation in the gel region is more and more common, and the variation of the electrophoretic band in the gel region where the glycinin A and B subunits are located is also observed. Each produces a unique electrophoretic spectrum that distinguishes each other. According to the type of seed protein electrophoresis, nine perennial species can be divided into three groups. The first group only G. tomentella a, its electrophoresis and soja sub-genus closest. The second group includes G.cladestina, G.canescens G.latrobeana and G.argyraea, and the third group consists of G.tabacina, G.latifolia, G.cyrtola6a and G.falcata. The G.wightii electrophoresis is the most unique. A relatively small variation in seed protein was observed between the pools in most species.