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目的 初步探讨模拟微重力对许旺细胞三维培养的影响 .方法 分别把粘附在聚羟基乙酸支架上生长3、7、10及 14天的许旺细胞和新鲜分离的原代许旺细胞悬液连同聚羟基乙酸支架送入转壁式生物反应器 (RWVB)中旋转培养 5天 ,观察细胞在支架上的生长情况及超微结构的改变 .结果 粘附在支架上的许旺细胞进入模拟微重力环境后细胞变圆变小 ,核染色质聚集 ,细胞器水肿扩张 ,核 /浆比例明显增大 ,微丝微管松散紊乱 ,细胞在 3天内快速凋亡或碎裂溶解 ;尚未贴壁的原代许旺细胞进入模拟微重力环境后在支架表面均匀贴壁生长、增殖 ,蛋白合成活跃 ,个别细胞内可见髓鞘样结构 ,5天内未见细胞死亡现象 .结论 贴壁生长 3～ 14天的许旺细胞进入模拟微重力环境后表现为快速凋亡、溶解 ,而未贴壁的原代许旺细胞进入模拟微重力环境后则功能活跃表达及快速增殖 ,可用于构建比较理想的许旺细胞三维培养体系 .
Objective To investigate the effects of simulated microgravity on the three-dimensional culture of Schwann cells.Methods Schwann cells adhered to polyglycolic acid scaffolds for 3, 7, 10 and 14 days respectively were cultured with freshly isolated primary Schwann cells Together with polyglycolic acid scaffolds, were introduced into a transwell bioreactor (RWVB) and cultured for 5 days. The growth of the cells on the scaffolds and ultrastructural changes were observed. RESULTS: Schwann cells adhering to the scaffolds entered the simulated micro After the gravitational environment, the cells became round and smaller, the chromatin gathered, the edema of organelles expanded, the ratio of nuclear / plasma increased obviously, the microfilament microtubules were loosely disrupted, and the cells rapidly apoptosis or fragmentation dissolved within 3 days; On the surface of scaffolds, the Schwann cells appeared adherent growth, proliferation and protein synthesis after entering the simulated microgravity environment, and the myelin-like structures were observed in individual cells and no cell death was observed in 5 days.Conclusion The growth of adherent cells was observed in 3 ~ 14 days Schwann cells showed rapid apoptosis and lysis after entering the simulated microgravity environment, while the non-adherent primary Schwann cells entered the simulated microgravity environment, and then they were active and rapidly proliferating , Can be used to build a more ideal three-dimensional culture system of Schwann cells.