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目的 探讨卵巢摘除与血清肿瘤坏死因子 α(TNF α)、转化生长因子 β1(TGF β1)浓度的关系。 方法 30只SD大鼠随机分为模型Ⅰ (OVXⅠ )组、模型Ⅱ (OVXⅡ )组和假手术组 ,OVXⅠ、OVXⅡ组大鼠卵巢切除 ,术后 12和 2 4周运用酶联免疫法检测大鼠血清中TNF α、TGF β1浓度的变化。 结果 大鼠去卵巢后当雌二醇 (E2 )水平下降时 ,血清TNF α浓度〔(5 3 88± 16 35 )ng/L〕升高 ,而TGF β1含量〔(6 78± 0 75 ) μg/L〕下降 ,与假手术组比差异有显著性 (P <0 0 5或P <0 0 1) ;相关性分析显示 ,E2 与TNF α呈负相关 (r=- 0 384 ,P =0 0 5 ) ,而与TGF β1正相关 (r=0 75 0 ,P =0 0 0 1) ,两细胞因子之间亦呈负相关 (r=- 0 4 6 5 ,P =0 0 5 )。 结论 E2 对TNF α有抑制作用 ,但却能促进TGF β1的分泌 ,TNF α含量的增加和TGF β1水平的降低 ,均能刺激骨的吸收活动 ,导致骨质疏松症的发生。
Objective To investigate the relationship between ovarian removal and serum levels of tumor necrosis factor α (TNF α) and transforming growth factor β1 (TGF β1). Methods Thirty Sprague-Dawley rats were randomly divided into three groups: OVXⅠ, OVXⅡ, OVXⅡ, OVXⅠ, OVXⅡgroup, and ovariectomized OVXⅠ, OVXⅡgroup at 12 and 24 weeks after operation Changes of TNFα and TGFβ1 concentrations in serum of rats. Results When ovariectomized, the level of serum TNFα [(5388 ± 1635) ng / L〕 increased while the content of TGFβ1 〔((678 ± 755) μg / L], there was a significant difference between the two groups (P <0.05 or P <0.01). Correlation analysis showed that there was a negative correlation between E2 and TNFα (r = - 0 384, P = 0 (R = 0 75 0, P = 0 0 0 1). There was also a negative correlation between the two cytokines (r = - 0 465, P = 0 05). Conclusion E2 can inhibit the secretion of TNFα, but it can promote the secretion of TGFβ1. The increase of TNFα and the decrease of TGFβ1 can stimulate bone resorption and lead to osteoporosis.