应用实时定量PCR方法测定样品中转基因玉米Bt176的含量,并从多个方面来分析测量的不确定度。结果显示,A类不确定度为0.0003,B类不确定度为0.0007,合成不确定度为0.001,有效自由度veff为272,包含概率P为95%,扩展不确定度U=0.002,测量结果为(0.8±0.2)%。不确定度评估结果表明,测量不确定度的主要来源是微量液体转移量的偏差。 Real-time quantitative PCR method was used to determine the content of transgenic corn Bt176 in the sample, and the measurement uncertainty was analyzed from several aspects. The results showed that the uncertainty of class A was 0.0003, the uncertainty of class B was 0.0007, the uncertainty of synthesis was 0.001, the effective degree of freedom was veff was 272, the probability of inclusion was 95% and the uncertainty of expansion was U = 0.002. (0.8 ± 0.2)%. Uncertainty assessment results show that the main source of measurement uncertainty is the deviation of trace amount of liquid transfer.