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螺蛳是我国的一种特有腹足类,主要分布于云南省的高原湖泊中,是重要的经济螺类。为了探讨微囊藻毒素(MC)在螺蛳各组织中的分布及季节动态规律,于2008年1月及5～10月间按月采集滇池螺蛳样品,用酶联免疫检测法(Enzyme-Linked Immuno Sorbent Assay,ELISA)检测了肝脏、消化道、性腺及肌肉中MC含量。结果表明,MC在螺蛳的肝胰腺、消化道、性腺及肌肉中MC的含量均值分别为3.38±1.90、2.72±1.63、1.23±0.97和0.35±0.43μg/g DW;统计分析表明肝胰腺和消化道中MC含量无显著差异,但两者显著高于性腺,性腺显著高于肌肉。MC在各组织中含量最高月份出现在7月,含量最低月份出现在5月。相关性分析表明,性腺和肌肉组织中MC含量与周围环境水体中总MC含量之间显著正相关。按照WHO推荐的人体每日可允许摄入量TDI(≤0.04μg/kg人体重)标准进行推算发现,食用螺蛳可能存在潜在的风险。
Lo snail is an endemic gastropod in China, mainly located in plateau lakes in Yunnan Province, is an important economic snails. In order to investigate the distribution and seasonal dynamics of microcystin (MC) in various tissues of snail, snail samples from Dianchi Lake were collected on a monthly basis in January 2008 and May-October 2008 respectively. Enzyme-Linked Immunoassay Sorbent Assay, ELISA) to detect MC content in liver, digestive tract, gonad and muscle. The results showed that the average contents of MC in hepatopancreas, digestive tract, gonads and muscle of MC were 3.38 ± 1.90, 2.72 ± 1.63, 1.23 ± 0.97 and 0.35 ± 0.43μg / g DW, respectively. Statistical analysis showed that hepatopancreas and digestion Road, MC content was no significant difference, but both significantly higher than the gonads, gonads significantly higher than the muscle. The highest content of MC in all the tissues appeared in July and the lowest content in May appeared in May. Correlation analysis showed that there was a significant positive correlation between MC content in gonads and muscle tissue and total MC content in the surrounding water. According to WHO recommended daily allowable intake of human TDI (≤ 0.04μg / kg body weight) standard projections found eating snail may be potentially at risk.