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由流行性出血热病毒浙１０株感染沙鼠肾细胞，经０．０５％β－丙内酯灭活研制成的流行性出血热灭活疫苗，对８名志愿者免疫３针（０、７、２８天各注射１ｍｌ）。首针免疫后第２８天，血清用ＲＰＨＩＡ，ＥＬＩＳＡ和ＩＦＡ检测流行性出血热抗体，均全部阳转，空斑减少中和试验（ＰＲＮＴ）于第２８天有４人阳转，第４５天又有３人阳转，阳转率８７．５％，ＧＭＴ４０．０。于志愿免疫前及后７、１４、２８、４５天、３个月、０．５年、１年测定其３ＨＴｄＲ掺入法淋转试验显示：首针免疫后７天至３个月以内，其非特异性和（或）特异性Ｔ淋巴细胞转化程度低下，与免疫前相比有显著差异（Ｐ＜０．０５～０．００１）；特异性Ｂ淋巴细胞转化于免疫后１～１．５月明显增高，与免疫前相比Ｐ＜０．０５；非特异性的Ｔ、Ｂ淋巴细胞总的转化率亦以免后１～１．５月为高（Ｐ＜０．０５）。１年后，再加强免疫１针。加强免疫后１月测中和抗体、阳转率１００％、ＧＭＴ１１７．４，比初免高２倍，两者差异极为显著，特异与非特异性Ｔ淋巴细胞转化未再见抑制（Ｐ＞０．０５），而特异性Ｂ淋巴细胞转化极为明显增强（Ｐ＜０．００１）。
Infection of gerbil kidney cells by Zhejiang hemorrhagic fever virus Zhe 10 strain and epidemic hemorrhagic fever inactivated vaccine by inactivation of 0.05% β-propiolactone resulted in the immunization of 8 volunteers with 3 needles (0, 7 , 28 days each injection 1ml). On the 28th day after the first immunization, serum was detected by RPHIA, ELISA and IFA, all of them were positive and plaque reduction neutralization test (PRNT) had 4 positive animals on the 28th day. On the 45th day There are 3 positive Yang, positive conversion rate of 87.5%, GMT40.0. Before and after voluntary immunization 7,14,28,45 days, 3 months, 0.5 years, 1 year determination of 3HTdR incorporation lean test showed that: the first needle after immunization 7 days to 3 months, its Non-specific and (or) specific T lymphocyte transformation degree is low, compared with pre-immune significant difference (P <0.05 ~ 0.001); specific B lymphocyte transformation in immunity 1 ~ 1.5 months Significantly higher than that before immunization (P <0.05). The total non-specific T and B lymphocyte transformation rate was also higher after 1 to 1.5 months (P <0.05). 1 year later, and then strengthen the immune 1 needle. After boosting, the neutralizing antibody was detected in January. The rate of positive conversion was 100%, GMT117.4, which was 2 times higher than that of primary immunization. The difference between the two groups was extremely significant. The specific and nonspecific T lymphocyte transformation did not improve again (P> 0.05 ), While the conversion of specific B lymphocytes was significantly enhanced (P <0.001).