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目的:观察茶树菇多糖联合化疗对食管癌大鼠肿瘤坏死因子-α(TNF-α)和γ-干扰素(IFN-γ)的影响。方法:选用40只大鼠随机分为正常(正常对照)组,模型(模型对照)组,化疗(用替加氟)组,联合(替加氟联合茶树菇多糖)组。除正常组大鼠外,其他组大鼠皮下注射甲基戊基亚硝胺5 mg/kg,诱发食管癌模型,建模成功后各组治疗4周,记录大鼠的体重变化。用酶联免疫吸附(ELISA)法检测大鼠血清TNF-α和IFN-γ水平,采用免疫组化检测大鼠食管组织的TNF-α和IFN-γ表达情况。结果:在实验过程中,正常组大鼠体重呈逐步上升趋势,联合组大鼠体重呈缓慢上升趋势;而模型组和化疗组大鼠的体重变化不明显。联合组大鼠血清TNF-α水平明显低于模型组和化疗组,但高于正常组(P<0.05);而联合组大鼠血清IFN-γ水平明显高于模型组和化疗组,而低于正常组(P<0.05)。联合组大鼠的TNF-α阳性表达率低于模型组和化疗组,高于正常组;而IFN-γ阳性表达率高于模型组和化疗组,低于正常组(P<0.05)。结论:茶树菇多糖联合化疗可调节食管癌大鼠的免疫功能。其机制可能与下调TNF-α水平和上调IFN-γ水平有关。
Objective: To observe the effect of Agrocybegurnus polysaccharide combined with chemotherapy on tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ) in esophageal cancer rats. Methods: Forty rats were randomly divided into normal group, model group, chemotherapy group and tegafur group. Rats in other groups were injected subcutaneously with methyl pentylnitrosamine (5 mg / kg) to induce esophageal cancer model except rats in normal group. After successful modeling, the rats in each group were treated for 4 weeks and the body weight of rats were recorded. The levels of TNF-α and IFN-γ in serum were detected by enzyme-linked immunosorbent assay (ELISA), and the expressions of TNF-α and IFN-γ in esophageal tissues were detected by immunohistochemistry. Results: During the experiment, the body weight of rats in normal group showed a gradual upward trend. The body weight of rats in combination group showed a slowly increasing trend. However, the body weight of rats in model group and chemotherapy group did not change significantly. The level of TNF-α in the combined group was significantly lower than that in the model group and the chemotherapy group (P <0.05), while the level of IFN-γ in the combined group was significantly higher than that in the model group and the chemotherapy group In the normal group (P <0.05). The positive rate of TNF-α in the combination group was lower than that in the model group and the chemotherapy group, but higher than that in the normal group and the model group (P <0.05). Conclusion: Chaxingu polysaccharide combined with chemotherapy can regulate the immune function of esophageal cancer rats. The mechanism may be related to down-regulating the level of TNF-α and up-regulating the level of IFN-γ.