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水稻抗旱性研究是当前水稻研究的热点。采用农杆菌介导法将IPT基因导入籼稻IR64,并获得了254株再生植株,并对其进行PCR检测和Southern杂交分析,确定目的基因已整合到水稻中。选择两个单拷贝的转基因株系和一个野生型株系在开花期对其进行干旱胁迫,在同等水平干旱胁迫条件下,IPT转基因植株的细胞分裂素含量和叶绿素含量明显高于野生型,叶片温度稍微低于野生型,且转基因植株茎叶衰老延缓,这些结果表明IPT基因在抗旱育种及机理研究方面有积极的研究意义。
Research on drought resistance in rice is a hot issue in rice research. IPT gene was introduced into IR64 by Agrobacterium tumefaciens method and 254 regenerated plants were obtained. PCR and Southern hybridization analysis were carried out to confirm that the target gene has been integrated into rice. Two single-copy transgenic lines and one wild-type strain were selected to be drought-stressed at flowering stage. Under the same level of drought stress, the cytokinin content and chlorophyll content of IPT transgenic plants were significantly higher than those of wild type and leaves The temperature was slightly lower than wild type, and the senescence of stem and leaf of transgenic plants was delayed. These results indicated that IPT gene has a positive research significance in drought resistance breeding and mechanism research.