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目的探讨人类白细胞抗原(human leukocyte antigen,HLA)-Ⅰ类基因A,B,C位点多态性与河南地区儿童急性淋巴细胞白血病发生的相关性。方法采用序列特异性聚合酶链式反应方法对河南地区98例急性淋巴细胞白血病患儿(观察组)和373例正常对照个体的(对照组)HLA-A,B,C位点的多态性进行检测。结果 2组受试者共检出HLA-A等位基因型16种,HLA-B等位基因型37种,HLA-C等位基因型15种;其中观察组检出HLA-A,B,C等位基因型分别13,26,13种,而对照组分别检出15,36,14种;HLA-B*07在观察组中的频率0.015明显低于对照组的频率0.056(P=0.026,OR=0.261,95%CI:0.080～0.850,χ2=8.734);除HLA-B*07外,HLA-A,B,C其他各等位基因在观察组和对照组中分布差异无统计学意义(P>0.05)。结论 HLA-B*07在河南儿童急性淋巴细胞白血病发生中可能起保护作用。
Objective To investigate the association of human leukocyte antigen (HLA) -Ⅰtype A, B and C polymorphisms with the occurrence of acute lymphoblastic leukemia in children in Henan Province. Methods The sequence-specific polymerase chain reaction (PCR-RFLP) was used to detect HLA-A, B and C loci in 98 children with acute lymphoblastic leukemia (observation group) and 373 normal controls (control group) Test. Results A total of 16 HLA-A alleles, 37 HLA-B alleles and 15 HLA-C alleles were detected in the two groups. HLA-A, B, C allele were 13, 26, and 13, respectively, while 15, 36, 14 were detected in the control group. The frequency of HLA-B * 07 in the observation group was significantly lower than that of the control group , OR = 0.261, 95% CI: 0.080-0.850, χ2 = 8.734). The distribution of other alleles of HLA-A, B and C except HLA-B * 07 in observation group and control group showed no statistical difference Significance (P> 0.05). Conclusion HLA-B * 07 may play a protective role in the development of childhood acute lymphoblastic leukemia in Henan.