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研究利用前人开发的与玉米抗丝黑穗病主效QTL(bin2.09)紧密连锁的分子标记,在明确标记在bin2.09区域位置基础上,对不同来源的37份自交系进行抗感区分。结果表明,各标记在不同血缘自交系与黄早四抗病近等基因系间的区分效率有较大差异,其中,STS标记MZA6393在所有感病自交系与黄早四抗病近等基因系之间的区分效率最高,为75.7%dCAPS标记LSdCAP3与LSdCAP2的区分效率次之;多数标记对SPT血缘自交系的区分效果较好,且各标记对高感自交系的区分效果优于感病自交系,并最终预测由dCAPS标记LSdCAP2和STS标记MZA6393界定的片段可能为玉米抗丝黑穗病主效功能片段。
In this study, molecular markers closely linked to QTLs for maize head smut resistance were developed, and 37 inbred lines from different sources were screened on the basis of clearly labeled positions in bin 2.09 A sense of distinction. The results showed that there was a great difference in the efficiency of distinguishing the markers between the near-isogenic lines of Huang Zaisi and different lineages of inbred lines. Among them, the STS marker MZA6393 had nearly the same disease resistance in all susceptible inbred lines The segregation efficiency was the highest among 75.7% dCAPS markers, LSdCAP3 and LSdCAP2. Most markers were more discriminative to SPT inbred lines, and the markers were highly discriminative in high susceptible inbred lines In susceptible inbred lines, the fragment predicted by dCAPS marker LSdCAP2 and STS marker MZA6393 could be the main functional fragment of maize head smut resistance.