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目的 研究在模拟失重大鼠肺组织中一氧化碳表达的变化 ,探讨其在失重时肺组织损伤中的作用。 方法 采用尾部悬吊模拟失重 ,分为悬吊 7d和 2 1d及相应对照 4组 ,每组 10只健康雄性 SD大鼠 ,共 4 0只大鼠。并采用原位杂交技术检测肺组织诱导型血红素氧合酶 (HO- 1) m RNA表达情况。 结果 同正常对照组相比 ,7d尾悬吊模拟失重大鼠肺组织 HO- 1m RNA表达水平明显增高 ,且具有统计学意义 (P<0 .0 1) ,2 1d悬吊组模拟失重大鼠肺组织 HO- 1m RNA表达水平仍显著增高 (P<0 .0 1)。 结论 在模拟失重条件下肺组织一氧化碳的表达水平增高。
Objective To study the changes of carbon monoxide expression in lung tissue of simulated weightless rats and its role in lung injury during weightlessness. Methods The tail suspension was used to simulate the weightlessness. The rats were divided into 4 groups (7 days and 21 days after the suspension) and 4 control groups. There were 10 healthy male SD rats in each group, 40 rats in total. In situ hybridization was used to detect the expression of induced heme oxygenase (HO-1) m RNA in lung tissue. Results Compared with the normal control group, the HO-1mRNA expression in the lungs of 7 days tail-suspended mimic rats was significantly increased (P <0.01), and the weight-loss rats HO-1m RNA expression level in lung tissue was still significantly higher (P <0.01). Conclusion The expression of carbon monoxide in lung tissue under simulated weightlessness increased.