背景:川芎嗪具有降低庆大霉素的耳毒性作用,但川芎嗪能否通过增加耳蜗热休克蛋白70表达,从而拮抗庆大霉素的耳蜗毒性作用。目的:应用免疫组化及图像分析技术并结合听性脑干反应测试,观察川芎嗪对庆大霉素耳中毒豚鼠耳蜗热休克蛋白70表达的影响。设计:随机对照实验,直线相关分析。单位:沈阳医学院生理教研室和中国医科大学听力研究室。材料:选用清洁级耳郭反射灵敏的健康白色红目豚鼠40只,体质量200～250g,雌雄不拘。随机分为庆大霉素组、川芎嗪+庆大霉素组、川芎嗪组、正常对照组,每组10只。方法:川芎嗪+庆大霉素组每日左侧腹腔注射川芎嗪注射液140m g/kg,同时在右侧腹腔注射硫酸庆大霉素注射液100m g/kg。庆大霉素组:每日腹腔注射硫酸庆大霉素注射液100mg/kg。川芎嗪组每日腹腔注射川芎嗪注射液140m g/kg。正常对照组每日腹腔注射生理盐水2.5m L /kg,各组皆连续用药10d。各组动物混合饲养,每日测量体质量调整药量。在用药前和停药后分别检测各组大鼠听性脑干反应阈值,停药处死后应用SABC 免疫组化和图像分析技术,观察各组豚鼠耳蜗热休克蛋白70表达情况。主要观察指标:①各组大鼠听性脑干反应阈值。②各组大鼠耳蜗热休克蛋白70表达。结果:①豚鼠听性脑干反应阈值:川芎嗪+庆大霉素组、庆大霉素组均明显高于正常对照组犤(21.09±4.50)dBnH L,(36.55±6.13)dBnH L,(2.50±2.75)dBnHL,t=15.764～22.665,P <0.001犦。川芎嗪+庆大霉素组明显低于庆大霉素组(t=9.092,P <0.001)。②豚鼠耳蜗各部位热休克蛋白70表达:庆大霉素组耳蜗Corti’s器、血管纹和螺旋韧带、螺旋缘、螺旋神经节4个部位细胞热休克蛋白70阳性反应产物平均灰度值低于正常对照组犤(88.24±4.34,96.85±1.05;121.24±0.92,128.76±1.59;96.15±1.10,98.78±0.54;117.73±1.18,120.51±0.80),(t=6.097～18.307,P <0.001)犦。川芎嗪+庆大霉素组耳蜗Corti’s 器、血管纹和螺旋韧带、螺旋缘、螺旋神经节4个部位细胞热休克蛋白70阳性反应产物平均灰度值明显高于庆大霉素组(92.53±2.25,88.24±4.34;125.20±1.43,121.24±0.92;98.71±0.91,96.15±1.10;118.91±0.46,117.73±1.18,t=3.925～10.415,P <0.001)。③各组各部位热休克蛋白70阳性反应产物平均灰度值与听性脑干反应阈值高度相关(r=-0.8141～-0.9841,P <0.001)。结论:应用川芎嗪注射液可降低听性脑干反应阈值和庆大霉素中毒耳蜗热休克蛋白70的表达,以减轻庆大霉素的耳毒性损伤,从而改善听功能。 BACKGROUND: Tetramethylpyrazine has been shown to reduce the ototoxicity of gentamycin, but whether tetramethylpyrazine can increase the expression of heat shock protein 70 in the cochlea and antagonize the cochlear toxicity of gentamicin. OBJECTIVE: To investigate the effect of tetramethylpyrazine on gentamicin ototoxicity in the cochlea heat shock protein 70 expression in guinea pigs using immunohistochemical and image analysis techniques combined with auditory brainstem response test. Design: Randomized controlled trials, linear correlation analysis. Unit: Department of Physiology, Shenyang Medical College and Department of Hearing, China Medical University. MATERIALS: A total of 40 healthy white red-eyed guinea pigs with clean auricular reflex were used, and the body mass was 200-250 g, either male or female. They were randomly divided into gentamicin group, ligustrazine + gentamicin group, ligustrazine group, and normal control group, 10 in each group. METHODS: Ligustrazine plus gentamycin group received intraperitoneal injection of Ligustrazine injection 140m g/kg on the left side of the abdominal cavity, and gentamicin sulfate 100m g/kg on the right side. Gentamicin group: Daily intraperitoneal injection of gentamicin sulfate 100 mg/kg. Ligustrazine group was given intraperitoneal injection of Ligustrazine injection 140m g/kg. The normal control group received intraperitoneal injection of 2.5 m L/kg physiological saline every day, and each group received continuous administration for 10 days. Each group of animals was raised and mixed and daily body weight was adjusted. The thresholds of auditory brainstem responses in rats of each group were examined before and after drug withdrawal. SABC immunohistochemistry and image analysis techniques were used to observe the expression of heat shock protein 70 in the guinea pig cochlea. MAIN OUTCOME MEASURES: 1 The threshold of auditory brainstem response in each group. 2 The expression of heat shock protein 70 in the cochlea of ​​rats in each group. RESULTS: 1The auditory brainstem response threshold in guinea pigs was significantly higher in the ligustrazine + gentamicin group and the gentamicin group than in the normal control group (21.09 ± 4.50) dBnH L, (36.55 ± 6.13) dBnH L, ( 2.50±2.75) dBnHL, t=15.764 to 22.665, P<0.001犦. Ligustrazine + gentamicin group was significantly lower than gentamicin group (t = 9.092, P <0.001). 2 Expression of heat shock protein 70 in various parts of the cochlea of ​​guinea pigs: The average gray value of HSP70 positive reaction product in the gentamicin cochlea organ Corti’s apparatus, stria vascularis and spiral ligament, spiral edge, spiral ganglion The control group had 犤(88.24±4.34, 96.85±1.05; 121.24±0.92, 128.76±1.59; 96.15±1.10, 98.78±0.54; 117.73±1.18, 120.51±0.80), (t=6.097 to 18.307, P<0.001). The average gray value of HSP70 positive reaction product in the four parts of the Corti’s apparatus, stria vascularis, spiral ligament, spiral edge, and spiral ganglion in the tetramethylpyrazine + gentamicin group was significantly higher than that of the gentamicin group (92.53± 2.25, 88.24±4.34; 125.20±1.43, 121.24±0.92; 98.71±0.91, 96.15±1.10; 118.91±0.46, 117.73±1.18, t=3.925 to 10.415, P<0.001). 3 The average gray value of HSP70 positive reaction products in each group was highly correlated with auditory brainstem response threshold (r=-0.8141--0.9841, P < 0.001). Conclusion: The application of tetramethylpyrazine injection can reduce the threshold of auditory brainstem response and the expression of heat shock protein 70 in cochlea of ​​gentamycin poisoning, so as to reduce the ototoxicity damage of gentamicin and improve the auditory function.