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Two new acetylated kaempferol glycosides were isolated from the seeds of Camellia semiserrata Chi, their structures were elucidated as kaempferol-3-O-[(3-0-acetyl)-α-L-rharnnopyranosyl(1 → 3)(4-O-acetyl)-α-L-rhamnopyranosyl(l →6)-O-D-gluco-pyranoside] (1) and kaempferol-3-O-[(2-O-acetyl)-α-L-rhamnopyranosyl (1 → 3)(4-Oacetyl)-α-L-rhamnopyranosyl(l → 6)-β-d-gluco-pyranoside] (2) by spectral experiments (including ESI-MS, ID- and 2D-NMR). .