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为探讨深圳市一般人群中HGV和TTV感染状况及其影响因素。方法采用随机抽样法选取研究对象 ,对HGV感染的检测先用ELISA法检测样本中的抗 -HGV ,对其中阳性样本再用反转录PCR(RT -PCR)进行检测 ;TTVDNA则采用巢式PCR方法检测。结果表明 ,深圳市一般人群中HGVRNA和TTVDNA阳性率分别为 2 33%和 14 77% ,男女阳性率HGVRNA为 2 4 5%和2 2 0 % ,TTVDNA阳性率为 17 86%和 12 0 %。年龄组间HGVRNA及TTVDNA阳性率差异均无显著性 ;单因素和Logistic回归分析未显示肝炎病史、近期手术史、注射史、拔牙史及乙型肝炎疫苗接种史等因素与HGV及TTV感染有关 ;HBsAg、抗 -HBS和抗 -HBc与HGV及TTV感染无统计学意义。不同职业人群中HGVRNA阳性率以中学生和教师较高 ;TTVDNA阳性率则以税务干部和教师高于其他人群 ,因此证明 ,深圳市一般人群中HGV和TTV感染率较高 ,但其流行因素有待进一步研究
To explore the general population of Shenzhen HGV and TTV infection and its influencing factors. Methods The subjects were selected by random sampling method. The anti-HGV in samples was detected by ELISA and the positive samples were detected by reverse transcription-polymerase chain reaction (RT-PCR). The nested PCR Method detection. The results showed that the positive rates of HGV RNA and TTVDNA in the general population were 23.3% and 14.77% respectively in Shenzhen. The positive rates of male and female HGVRNA were 24.5% and 220.0%, respectively. The positive rates of TTVDNA were 1786% and 120%. The positive rates of HGV RNA and TTVDNA were not significantly different among the age groups. The single factor and Logistic regression analysis did not show the history of hepatitis, recent surgery history, injection history, history of tooth extraction and history of hepatitis B vaccination and other factors related to HGV and TTV infection; HBsAg, anti-HBS and anti-HBc with HGV and TTV infection was not statistically significant. The prevalence of HGVRNA in different occupational groups was higher among middle school students and teachers; the positive rate of TTVDNA was higher than that of other cadres in tax cadres and teachers, and therefore proved that the prevalence of HGV and TTV were higher in the general population in Shenzhen, but the epidemic factors needed further the study