真菌的单孢子分离和培养是菌种纯化、定向培育和细胞杂交的重要手段。单孢子分离方法很多,有的方法比较准确和现代化,但设备要求较高,如使用显微操作器,价钱昂贵且尚需进口。而另一些方法如稀释法、滴定法等较简单,但其准确性常因使用者的习惯和熟练程度而异。我们在茯苓单孢子培养和交配试验中,采用的微型玻璃毛细管针吸孢法,具有分离单孢 Isolation and culture of fungal spores is an important means of bacterial culture, targeted cultivation and cell hybridization. There are many methods of single spore separation, some methods are more accurate and modern, but the equipment is more demanding, such as the use of micro-manipulator, expensive and still need to import. Other methods, such as dilution and titration, are simpler, but their accuracy often varies depending on the user’s habits and proficiency. We are in the Poria culture and mating test, the use of micro-glass capillary needle suction method, with isolated spores